Goat Prostaglandin D2 Synthase, Brain ELISA Kit | PGD2S elisa kit

Goat Prostaglandin D2 Synthase, Brain ELISA Kit

Cat. Num. AAA93624

• Gene Names: PTGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• Reactivity: Goat
• Synonyms: Prostaglandin D2 Synthase, Brain; N/A; Goat Prostaglandin D2 Synthase, Brain ELISA Kit; PGD2S elisa kit

• Reactivity: Goat
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.

• Intended Uses: This PGD2S ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat PGD2S. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for PGD2S elisa kit

Principle of the Assay: PGD2S ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGD2S antibody and an PGD2S-HRP conjugate. The assay sample and buffer are incubated together with PGD2S-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGD2S concentration since PGD2S from samples and PGD2S-HRP conjugate compete for the anti-PGD2S antibody binding site. Since the number of sites is limited, as more sites are occupied by PGD2S from the sample, fewer sites are left to bind PGD2S-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGD2S concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PGD2S elisa kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 32171249
• NCBI GeneID: 5730
• NCBI Accession #: NP_000945.3
• NCBI GenBank Nucleotide #: NM_000954.5
• UniProt Accession #: P41222; Q5SQ10; Q7M4P3; Q9UC22; Q9UCC9; Q9UCD9; B2R727
• Molecular Weight: 21,029 Da
• NCBI Official Full Name: prostaglandin-H2 D-isomerase
• NCBI Official Synonym Full Names: prostaglandin D2 synthase 21kDa (brain)
• NCBI Official Symbol: PTGDS
• NCBI Official Synonym Symbols: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• NCBI Protein Information: prostaglandin-H2 D-isomerase; cerebrin-28; PGD2 synthase; beta-trace protein; prostaglandin D synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; lipocalin-type prostaglandin-D synthase
• UniProt Protein Name: Prostaglandin-H2 D-isomerase
• UniProt Gene Name: PTGDS
• UniProt Synonym Gene Names: PDS; PGD2 synthase; PGDS; PGDS2
• UniProt Entry Name: PTGDS_HUMAN