Application Data
(This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.746)
Phl p 5b recombinant protein
Recombinant allergen Phl p 5b for Phleum pratense (Timothy grass pollen)
Applications
ELISA
Purity
15% SDS-PAGE
Synonyms
Phl p 5b; N/A; Recombinant allergen Phl p 5b for Phleum pratense (Timothy grass pollen); Phl p 5b recombinant protein
Host
E Coli
Purity/Purification
15% SDS-PAGE
Form/Format
Dry powder (lyophilized)
Concentration
1.44 mg/ml (varies by lot)
Applicable Applications for Phl p 5b recombinant protein
ELISA
Organism
Plehum Pratense
Storage Buffer
50 mM MES pH 6.5 and 0.1 M KCl
Quality Control
1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Application Data
(This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.746)
Application Data
(This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.746)
SDS-PAGE
(Figure 1. SDS-PAGE analysis (15%) of 4 ul of recombinant allergen. Purity is > 95% as determined by gel electrophoresis.)
SDS-PAGE
(Figure 1. SDS-PAGE analysis (15%) of 4 ul of recombinant allergen. Purity is > 95% as determined by gel electrophoresis.)
Related Product Information for Phl p 5b recombinant protein
Recombinant Allergen: Phleum pratense Phl p 5 is one of major pollen allergen of timothy grass and accounts for IgE binding in up to 60% of patients (Vrtala et al., 1993). Phl p 5b is one of the isoallergenic groups of Phl p 5 (van Neerven et al., 1992).
Description: The Phleum pratense major isoallergen Phl p 5b has been prepared as the recombinant mature allergen fused to a his-tag.
Description: The Phleum pratense major isoallergen Phl p 5b has been prepared as the recombinant mature allergen fused to a his-tag.
References
Vrtala, S., Sperr, W.R., Reimitzer, I., van Ree, R., Laffer, S., Muller, W.D., Valent, P., Lechner, K.,Rumpold, H., Kraft, D. cDNA cloning of a major allergen from timothy grass (Phleum pratense) pollen; characterization of the recombinant Phl pV allergen. 1993. J. Immunol. 151, 4773-4781.
van Neerven RJ1, Wikborg T, Lund G, Jacobsen B, Brinch-Nielsen A, Arnved J, Ipsen H. Blocking antibodies induced by specific allergy vaccination prevent the activation of CD4+ T cells by inhibiting serum-IgEfacilitated allergen presentation. 1999. J Immunol. 163:2944-52.
Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.
van Neerven RJ1, Wikborg T, Lund G, Jacobsen B, Brinch-Nielsen A, Arnved J, Ipsen H. Blocking antibodies induced by specific allergy vaccination prevent the activation of CD4+ T cells by inhibiting serum-IgEfacilitated allergen presentation. 1999. J Immunol. 163:2944-52.
Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.
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Product Notes
The Phl p 5b (Catalog #AAA72866) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phl p 5b can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the Phl p 5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phl p 5b, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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