Application Data
(ADP-Glo assay for PI3Ka/p85 Complex activity 0.125 ug/ul PI-3 Kinase Lipid Substrate was incubated with different concentrations of PI3Ka/p85 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
PI3Kalpha/p85 recombinant protein
Recombinant PI3Kalpha/p85 protein complex
Synonyms
PI3Kalpha/p85; N/A; Recombinant PI3Kalpha/p85 protein complex; Phosphoinositide 3-kinase; PI3K; P110-Alpha; CLAPO; CLOVE; CCM4; MCAP; MCM; PI3Kalpha/p85 recombinant protein
Host
Baculovirus
Form/Format
Recombinant PI3Ka/p85 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant PI3Ka/p85 Complex that includes full length human PI3Ka protein (accession number NP_006209.2) with an N-terminal DYKDDDDK tag and full length human p85 protein (accession number NP_852664.1) without tag was expressed in Hi5 cells. The molecular weights of PI3Ka and p85 are 125.6kDa and 83.6kDa, respectively.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(ADP-Glo assay for PI3Ka/p85 Complex activity 0.125 ug/ul PI-3 Kinase Lipid Substrate was incubated with different concentrations of PI3Ka/p85 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
Application Data
(ADP-Glo assay for PI3Ka/p85 Complex activity 0.125 ug/ul PI-3 Kinase Lipid Substrate was incubated with different concentrations of PI3Ka/p85 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE
(Recombinant PI3Ka/p85 protein 10% SDS-PAGE Coomassie staining MW: PI3Ka: 125.6kDa, p85: 83.6kDa Purity: >=90%)
SDS-PAGE
(Recombinant PI3Ka/p85 protein 10% SDS-PAGE Coomassie staining MW: PI3Ka: 125.6kDa, p85: 83.6kDa Purity: >=90%)
Related Product Information for PI3Kalpha/p85 recombinant protein
Phosphoinositide 3-kinase (PI3K) family of lipid kinases are divided into three major classes based on primary sequence, substrate preference, and regulation. While class IA PI3Ks are heterodimeric enzymes composed of a catalytic subunit (p110a, p110b, or p110d) complexed with one of five regulatory subunits (p85a, p55a, p50a, p85b, or p55g), the classIB enzyme is a dimer made of p110g catalytic subunit and p101or p84 regulatory subunit. The class I catalytic subunit polypeptides p110a, p110b, p110d, and p110g are encoded by PIK3CA, PIK3CB, PIK3CD, and PIK3CG, respectively. The regulatory subunits are encoded by five genes: PIK3R1 codes p85a, p55a, and p50a; PIK3R2 codes p85b; PIK3R3 codes p55g; PIK3R5 codes p101; and PIK3R6 codes p84. PI3K is a lipid and protein kinase that can be activated by receptor tyrosine kinases, cytokine receptors, chemokine G protein–coupled receptors, or oncogenes, including RAS. PI3K converts phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-triphosphate (PIP3), which leads to activation of pyruvate dehydrogenase kinase 1 (PDK1) and the serine/threonine kinase AKT (PKB) (7). AKT has many downstream effectors, which regulate the cell cycle, apoptosis, protein translation, autophagy, and metabolism. Most cytokines and RTK ligands signal through class IA PI3K.
Product Categories/Family for PI3Kalpha/p85 recombinant protein
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Product Notes
The PI3Kalpha/p85 (Catalog #AAA60419) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PI3Kalpha/p85, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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