Standard Curve (Sample)
Pig Complement Component 3a (C3a) CLIA Kit | C3a clia kit
Pig Complement Component 3a (C3a) CLIA Kit
Gene Names
C3; C3d
Reactivity
Pig
Synonyms
Complement Component 3a (C3a); N/A; Pig Complement Component 3a (C3a) CLIA Kit; C3a CLIA kit (Pig); Complement Component 3a; C3a clia kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Component 3a (C3a). No significant cross-reactivity or interference between Complement Component 3a (C3a) and analogues was observed.
Reactive with Pig C3a/Complement Component 3a
Reactive with Pig C3a/Complement Component 3a
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and other biological fluids
Detection Range
31.2-2,000pg/mL
Sensitivity
11.2pg/mL
Sample Size
100uL
Detection Method
Chemiluminescence
Featured Series
SE kit
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3a (C3a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3a (C3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3a (C3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Function
Detects protein (regular version)
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 3a (C3a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 3a (C3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 3a (C3a) level in the sample or standard.;
Research Area
Infection immunity, Immune molecule, Autoimmunity
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,807 Da
NCBI Official Full Name
complement C3
NCBI Official Symbol
C3
NCBI Official Synonym Symbols
C3d
NCBI Protein Information
complement C3
UniProt Protein Name
Complement C3
UniProt Gene Name
C3
UniProt Synonym Gene Names
C3bc; ASP
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Product Notes
The Pig C3a c3 (Catalog #AAA190102) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190102 CLIA Kit recognizes Pig C3a. It is sometimes possible for the material contained within the vial of "Complement Component 3a (C3a), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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