Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit | PIK3CA elisa kit

Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) ELISA Kit

Cat. Num. AAA55406

• Gene Names: PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
• Reactivity: Human
• Synonyms: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; N/A; Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) ELISA Kit; PIK3CA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PIK3CA. No significant cross-reactivity or interference between Human PIK3CA and analogues was observed.
• Sequence Length: 1068
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.31 ng/mL - 20 ng/mL
• Sensitivity: 0.156 ng/mL (mean of 6 independent assays)

• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PIK3CA elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIK3CA in Human serum, plasma. An antibody specific for PIK3CA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIK3CA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3CA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3CA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: For the quantitative detection of Human Phosphatidylinositol-4,5- bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) concentration in serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 54792082
• NCBI GeneID: 5290
• NCBI Accession #: NP_006209.2
• NCBI GenBank Nucleotide #: NM_006218.3
• UniProt Accession #: P42336; Q14CW1; Q99762
• Molecular Weight: 124,284 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
• NCBI Official Symbol: PIK3CA
• NCBI Official Synonym Symbols: MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Gene Name: PIK3CA
• UniProt Synonym Gene Names: PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha