Application Data
PI3K recombinant protein
PI3K (p110 beta/p85 beta), Active
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA
Applications
Kinase Assay
Synonyms
PI3K; N/A; PI3K (p110 beta/p85 beta), Active; PI3K recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Sequence Positions
Full length
Sequence Length
5931
Applicable Applications for PI3K recombinant protein
Kinase Assay
Species
Human
Tag
HIS tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PI3K recombinant protein
Description: Recombinant full-length human p110 beta and human p85 beta were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Scientific Background: PI3K phosphorylate the 3-prime OH position of the inositol ring of inositol lipids which functions in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kDa catalytic subunit and an 85-kDa adaptor subunit (1). PI3K plays a critical role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. PI3K sustains alpha-2B-beta-3 integrin activation and stabilized platelet aggregation by regulating both integrin-dependent calcium flux and Gi activation of RAP1B (2).
Scientific Background: PI3K phosphorylate the 3-prime OH position of the inositol ring of inositol lipids which functions in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kDa catalytic subunit and an 85-kDa adaptor subunit (1). PI3K plays a critical role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. PI3K sustains alpha-2B-beta-3 integrin activation and stabilized platelet aggregation by regulating both integrin-dependent calcium flux and Gi activation of RAP1B (2).
Product Categories/Family for PI3K recombinant protein
References
1. Hu, P.et.al: Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. Molec. Cell. Biol. 13: 7677-7688, 1993. 2. ackson, S. P.et.al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~111 kDa; ~88 kDa
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
NCBI Official Symbol
PIK3CB
NCBI Official Synonym Symbols
PI3K; PIK3C1; P110BETA; PI3KBETA
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
UniProt Gene Name
PIK3CB
UniProt Synonym Gene Names
PIK3C1; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta
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Product Notes
The PI3K pik3cb (Catalog #AAA73062) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's PI3K can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the PI3K pik3cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3K, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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