Application Data
PI3K recombinant protein
PI3K (p120 gamma), Active
Gene Names
PIK3CG; PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
Applications
Kinase Assay
Synonyms
PI3K; N/A; PI3K (p120 gamma), Active; PI3K recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Sequence Positions
Full length
Sequence Length
7218
Applicable Applications for PI3K recombinant protein
Kinase Assay
Species
Human
Tag
HIS tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PI3K recombinant protein
Description: Recombinant full-length human PI3K (p120 gamma) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: PI3K (p120 gamma) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. PI3K (p120 gamma) is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, PI3K (p120 gamma) is thought to play a pivotal role in the regulation of cytotoxicity in NK cells (1). In addition, PI3K (p120 gamma) plays an inhibitory role in inflammatory mediator-induced human vascular smooth muscle cell calcification through regulating alkaline phosphatase expression (2).
Scientific Background: PI3K (p120 gamma) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. PI3K (p120 gamma) is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, PI3K (p120 gamma) is thought to play a pivotal role in the regulation of cytotoxicity in NK cells (1). In addition, PI3K (p120 gamma) plays an inhibitory role in inflammatory mediator-induced human vascular smooth muscle cell calcification through regulating alkaline phosphatase expression (2).
Product Categories/Family for PI3K recombinant protein
References
1. Jiang, K. et al: Pivotal role of phosphoinositide-3 kinase in regulation of cytotoxicity in natural killer cells. Nature Immun. 1: 419-425, 2000. 2. Patrucco, E.et al: PI3K-gamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell 118: 375-387, 2004.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121 kDa
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma (PIK3CG), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
NCBI Official Symbol
PIK3CG
NCBI Official Synonym Symbols
PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
UniProt Gene Name
PIK3CG
UniProt Synonym Gene Names
PI3-kinase subunit gamma; PI3K-gamma; PI3Kgamma; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; p110gamma
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Product Notes
The PI3K pik3cg (Catalog #AAA73064) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's PI3K can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the PI3K pik3cg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3K, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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