PI3K (p85 alpha) recombinant protein

PI3K (p85 alpha) recombinant protein

Cat. Num. AAA72905

• Gene Names: PIK3R1; p85; AGM7; GRB1; p85-ALPHA
• Applications: Western Blot
• Purity: >95%
• Synonyms: PI3K (p85 alpha); N/A; PI3K (p85 alpha) recombinant protein; PI3K catalytic Domain alpha; PIK3R1; GRB1; p85-ALPHA; PI3K (p85alpha) Protein; PI3K (p85a) Protein

• Host: Sf9 insect cells
• Purity/Purification: >95%
• Form/Format: Recombinant protein stored in 50mM Sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.25 mM DTT, 25% Glycerol.
• Concentration: 0.2 ug/uL (varies by lot)

• Applicable Applications for PI3K (p85 alpha) recombinant protein: WB (Western Blot), Kinase Assay
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; ; Ships with dry ice.

SDS-PAGE

(The purity of p85alpha was determined to be 95% by densitometry. Approx. MW 86 kDa)

Related Product Information for PI3K (p85 alpha) recombinant protein

Recombinant full-length human PI3K (p85alpha) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is (NM_181523).

Scientific Background: The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85alpha modulates the interaction between PI3K and platelet-derived growth factor receptor (1). Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) (2).

Product Categories/Family for PI3K (p85 alpha) recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

References

1. Skolnik, E. Y. Et al: Cloning of PI3-kinase associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. Cell 65: 83-90, 1991.
2. Simoncini, T. et al: Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase. Nature 407: 538-541, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 335057530
• NCBI GeneID: 5295
• NCBI Accession #: NP_852664.1
• NCBI GenBank Nucleotide #: NM_181523
• UniProt Accession #: P27986; Q15747; Q4VBZ7; Q53EM6; Q8IXA2; Q8N1C5; B3KT19; D3DWA0; E7EX19
• NCBI Official Full Name: Homo sapiens phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN