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product-image-AAA73873_SDS_PAGE13.gif SDS-PAGE (Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

PKC nu Recombinant Protein | PKCnu recombinant protein

PKC nu, Active

Applications
Western Blot
Synonyms
PKC nu; N/A; PKC nu, Active; EPK2; PRKCN; PKD3; nPKC-NU; PKCnu recombinant protein
Ordering
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Applicable Applications for PKCnu recombinant protein
WB (Western Blot), Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

product-image-AAA73873_SDS_PAGE13.gif SDS-PAGE (Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

product-image-AAA73873_ACTIVITY15.gif Activity (Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PKCnu recombinant protein
Recombinant full-length human PKCnu was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_005813.
PKCnu, also known as PKD3, is a members of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCnu is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKCmu (PKCnu) and 77. 4% similarity to mouse PKD (1). The PKCnu mRNA is ubiquitously expressed in various tissues and the gene is located between markers WI-9798 and D2S177 on chromosome 2p21 region.
References
1. Hayashi,A. et al: PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu. Biochim Biophys Acta. 1999 May 6;1450(1):99-106.

NCBI and Uniprot Product Information

NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~142kDa

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Product Notes

The PKCnu (Catalog #AAA73873) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PKC nu can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the PKCnu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC nu, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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