Standard Curve (Sample)
General uPA functional assay ELISA Kit | PLAU elisa kit
Active human uPA functional assay ELISA Kit
Gene Names
PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
Reactivity
General
Synonyms
uPA functional assay; N/A; Active human uPA functional assay ELISA Kit; PLAU elisa kit
Reactivity
General
Specificity
These studies are currently in progress. Please contact us for more information.
Form/Format
Complete Kit
Sequence Length
414
Samples
Human plasma
Sensitivity
The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty-two zero standard replicates (range OD450: 0.05-0.055) and calculating the corresponding concentration. The MDD was 0.015 ng/ml.
Intra-assay Precision
These studies are currently in progress. Please contact us for more information.
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PLAU elisa kit
Intended Uses: Human uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in human plasma. For research use only.
Principle of the Assay: Functionally active uPA present in plasma will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Latent or complexed uPA will not bind to the plate and will not be detected. Unbound uPA samples are washed away and an anti-uPA primary antibody is added. Excess primary antibody is washed away and bound antibody is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.
Background: Urokinase plasminogen activator, along with its receptor uPAR, initiates a proteolytic cascade that results in the conversion of plasminogen to plasmin [1]. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis [2,4]. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients [3]. Increased uPA activity may be an implication for the pathophysiology of endometriosis [5].
Principle of the Assay: Functionally active uPA present in plasma will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Latent or complexed uPA will not bind to the plate and will not be detected. Unbound uPA samples are washed away and an anti-uPA primary antibody is added. Excess primary antibody is washed away and bound antibody is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.
Background: Urokinase plasminogen activator, along with its receptor uPAR, initiates a proteolytic cascade that results in the conversion of plasminogen to plasmin [1]. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis [2,4]. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients [3]. Increased uPA activity may be an implication for the pathophysiology of endometriosis [5].
Product Categories/Family for PLAU elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,507 Da
NCBI Official Full Name
urokinase-type plasminogen activator isoform 2
NCBI Official Synonym Full Names
plasminogen activator, urokinase
NCBI Official Symbol
PLAU
NCBI Official Synonym Symbols
ATF; QPD; UPA; URK; u-PA; BDPLT5
NCBI Protein Information
urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
UniProt Protein Name
Urokinase-type plasminogen activator
UniProt Gene Name
PLAU
UniProt Synonym Gene Names
U-plasminogen activator; uPA
UniProt Entry Name
UROK_HUMAN
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Product Notes
The General PLAU plau (Catalog #AAA37743) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37743 ELISA Kit recognizes General PLAU. It is sometimes possible for the material contained within the vial of "uPA functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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