Standard Curve (Sample)
Human uPA ELISA Kit | PLAU elisa kit
Human uPA ELISA Kit
Gene Names
PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
Reactivity
Human
Applications
ELISA
Synonyms
uPA; N/A; Human uPA ELISA Kit; Urokinase-type plasminogen activator; U-plasminogen activator; PLAU elisa kit
Reactivity
Human
Specificity
The Human uPA ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human uPA proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
414
Samples
Culture Supernatants, Serum, Plasma
Sensitivity
< 7 pg/ml
Applicable Applications for PLAU elisa kit
ELISA (Sandwich ELISA)
Detection Method
Colorimetric
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PLAU elisa kit
Background: Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).
Principle of the Assay: The Human uPA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human uPA in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human uPA coated on a 96-well plate. Standards and samples are pipetted into the wells and uPA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human uPA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of uPA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human uPA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human uPA in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human uPA coated on a 96-well plate. Standards and samples are pipetted into the wells and uPA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human uPA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of uPA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,908 Da
NCBI Official Full Name
urokinase-type plasminogen activator isoform 2
NCBI Official Synonym Full Names
plasminogen activator, urokinase
NCBI Official Symbol
PLAU
NCBI Official Synonym Symbols
ATF; QPD; UPA; URK; u-PA; BDPLT5
NCBI Protein Information
urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
UniProt Protein Name
Urokinase-type plasminogen activator
UniProt Gene Name
PLAU
UniProt Synonym Gene Names
U-plasminogen activator; uPA
UniProt Entry Name
UROK_HUMAN
Similar Products
Product Notes
The Human PLAU plau (Catalog #AAA106083) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA106083 ELISA Kit recognizes Human PLAU. AAA Biotech's uPA can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the PLAU plau for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "uPA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.