Human Neuropathy target esterase ELISA Kit | PNPLA6 elisa kit
Human Neuropathy target esterase ELISA Kit
Reactivity
Human
Synonyms
Neuropathy target esterase; N/A; Human Neuropathy target esterase ELISA Kit; PNPLA6/NTE/Patatin-like phospholipase domain-containing protein 6; PNPLA6 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PNKD. No significant cross-reactivity or interference between PNKD and analogues was observed.
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PNKD were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PNKD were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for PNPLA6 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PNKD antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PNKD antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PNKD amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PNKD can be calculated.
