WB (Western Blot)
(Western Blot: The Cell lysatederived from HT-29 wereresolved onto 10% SDSPAGE, transferred onto NCmembrane, and immunoblotted by Rabbit anti 15-LOX2 at1:500. An immunoreactiveband around ~75 kDa wasobserved.)
Rabbit anti-Human 15-Lox-2 Polyclonal Antibody | anti-15-Lox-2 antibody
Rabbit Anti 15-Lox-2 Polyclonal Antibody
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, ELISA, Western Blot
Purity
The Rabbit IgG is purified by Epitope Affinity Purification
Synonyms
15-Lox-2, Antibody; Rabbit Anti 15-Lox-2 Polyclonal Antibody; Rabbit anti-15-LOX1 Polyclonal Antibody; arachidonate 15-lipoxygenase Type B; ALOX2; Rabbit anti-15-LOX-2; anti-15-Lox-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This antibody recognizes ~73kDa of 15-LOX2 protein. It reacts with human. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification
Form/Format
This affinity purified antibody is supplied in sterile phosphate-buffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-15-Lox-2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Preparation
A synthetic peptide corresponding to the internal sequence oh Human 15-LOX-2.
Positive Control
Lung Tissue
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
WB (Western Blot)
(Western Blot: The Cell lysatederived from HT-29 wereresolved onto 10% SDSPAGE, transferred onto NCmembrane, and immunoblotted by Rabbit anti 15-LOX2 at1:500. An immunoreactiveband around ~75 kDa wasobserved.)
WB (Western Blot)
(Western Blot: The Cell lysatederived from HT-29 wereresolved onto 10% SDSPAGE, transferred onto NCmembrane, and immunoblotted by Rabbit anti 15-LOX2 at1:500. An immunoreactiveband around ~75 kDa wasobserved.)
Related Product Information for anti-15-Lox-2 antibody
This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. Alternatively spliced transcript variants encoding different isoforms. 15-Lipoxygenase 2 (15-LOX2) is ahuman lipoxygenase that shows tissue-restricted expression in prostate, lung, skin, and cornea. The protein level and enzymatic activity of 15-LOX2 have been shown to be down-regulated in prostate cancers compared with normal and benign prostate tissues. The biological function of 15-LOX2 and the role of loss of 15-LOX2 expression in prostate tumorigenesis
References
Tang,S., Bhatia,B., Maldonado,C.J., Yang,P., Newman,R.A., Liu,J.,et al. Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. J. Biol. Chem. 277 (18), 16189-16201 (2002).
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Product Notes
The 15-Lox-2 (Catalog #AAA71353) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti 15-Lox-2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 15-Lox-2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the 15-Lox-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "15-Lox-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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