ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5mug anti-HIST1H4A (CSB-PA010429PA12hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
Rabbit anti-Human 2-hydroxyisobutyryl-HIST1H4A Polyclonal Antibody | anti-HIST1H4A antibody
2-hydroxyisobutyryl-HIST1H4A (K12) Antibody
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5mug anti-HIST1H4A (CSB-PA010429PA12hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5mug anti-HIST1H4A (CSB-PA010429PA12hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
IP (Immunoprecipitation)
(Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)Lane 1: Rabbit control IgG instead of CSB-PA010429PA12hibHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA010429PA12hibHU (3ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug)Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)Lane 1: Rabbit control IgG instead of CSB-PA010429PA12hibHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA010429PA12hibHU (3ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug)Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug))
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429PA12hibHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429PA12hibHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of CSB-PA010429PA12hibHU diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of CSB-PA010429PA12hibHU diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotDetected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4hAll lanes: HIST1H4A antibody at 1.25ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 12 kDaObserved band size: 12 kDa)
WB (Western Blot)
(Western BlotDetected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4hAll lanes: HIST1H4A antibody at 1.25ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 12 kDaObserved band size: 12 kDa)
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Product Notes
The HIST1H4A hist1h4a (Catalog #AAA235319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 2-hydroxyisobutyryl-HIST1H4A (K12) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 2-hydroxyisobutyryl-HIST1H4A can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HIST1H4A hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "2-hydroxyisobutyryl-HIST1H4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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