ICC (Immunocytochemistry)
(Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/mL.)
Rabbit anti-Human, Mouse 4E-BP1 Polyclonal Antibody | anti-4E-BP1 antibody
4E-BP1 Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
4E-BP1, Antibody; 4E-BP1 Antibody; initiation factor 4E binding protein 1; anti-4E-BP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
118
Applicable Applications for anti-4E-BP1 antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a 14 amino acid peptide from near the carboxy terminus of human 4E-BP1.
Target Name
4E-BP1
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ICC (Immunocytochemistry)
(Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/mL.)
WB (Western Blot)
(Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/mL.)
WB (Western Blot)
(Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/mL.)
Related Product Information for anti-4E-BP1 antibody
The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.
Product Categories/Family for anti-4E-BP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,580 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
UniProt Entry Name
4EBP1_HUMAN
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Product Notes
The 4E-BP1 eif4ebp1 (Catalog #AAA306996) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 4E-BP1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the 4E-BP1 eif4ebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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