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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Goat 5HT2A Receptor Polyclonal Antibody | anti-HTR2A antibody
Goat anti-5HT2A Receptor (aa296-309) Antibody
Gene Names
HTR2A; HTR2; 5-HT2A
Reactivity
Tested: HumanExpected from sequence similarity: Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
5HT2A Receptor, Antibody; Goat anti-5HT2A Receptor (aa296-309) Antibody; Goat Anti-HTR2A Antibody; HTR2A; 5-hydroxytryptamine (serotonin) receptor 2A; 5-HT2A; HTR2; 5-HT-2; 5-HT-2A; 5-HT2 receptor; 5-hydroxytryptamine receptor 2A; OTTHUMP00000018378; serotonin 5-HT-2A receptor; serotonin receptor 2A; 5-HT-2 antibody; 5-HT2 receptor antibody; 5-HT2A antibody; 5-HT-2A antibody; 5-hydroxytryptamine (serotonin) receptor 2A antibody; 5-hydroxytryptamine receptor 2A antibody; HTR2 antibody; HTR2A antibody; OTTHUMP00000018378 antibody; serotonin 5-HT-2A receptor antibody; serotonin receptor 2A antibody; 5HT2A Receptor; 5HT2A Receptor (aa296-309); anti-HTR2A antibody
Host
Goat
Reactivity
Tested: Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
QRSIHREPGSYTGR
Sequence Length
471
Applicable Applications for anti-HTR2A antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-QRSIHREPGSYTGR, from the internal region of the protein sequence according to NP_000612.1; NP_001159419.1.
Note
This antibody is expected to recognize both reported isoforms (NP_000612.1; NP_001159419.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IHC (Immunohiostchemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
IHC (Immunohiostchemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
IHC (Immunohistochemistry)
((2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
((2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
5-hydroxytryptamine receptor 2A isoform 1
NCBI Official Synonym Full Names
5-hydroxytryptamine receptor 2A
NCBI Official Symbol
HTR2A
NCBI Official Synonym Symbols
HTR2; 5-HT2A
NCBI Protein Information
5-hydroxytryptamine receptor 2A
UniProt Protein Name
5-hydroxytryptamine receptor 2A
UniProt Gene Name
HTR2A
UniProt Synonym Gene Names
HTR2; 5-HT-2; 5-HT-2A
UniProt Entry Name
5HT2A_HUMAN
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Product Notes
The HTR2A htr2a (Catalog #AAA61649) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-5HT2A Receptor (aa296-309) Antibody reacts with Tested: Human Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's 5HT2A Receptor can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the HTR2A htr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRSIHREPGS YTGR. It is sometimes possible for the material contained within the vial of "5HT2A Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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