IHC (Immunohistochemistry)
(Anti-5HT2A Receptor antibody, AAA11510, IHC(P)IHC(P): Rat Brain Tissue)
Rabbit 5HT2A Receptor Polyclonal Antibody | anti-HTR2A antibody
Anti-5HT2A Receptor antibody
Gene Names
HTR2A; HTR2; 5-HT2A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
5HT2A Receptor, Antibody; Anti-5HT2A Receptor antibody; 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled; 5-hydroxytryptamine receptor 2A; anti-HTR2A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na<.sub>2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Sequence Length
471
Applicable Applications for anti-HTR2A antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot (WB):
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Mouse, Rat
Predicted Species: Human
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Mouse, Rat
Predicted Species: Human
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HTR2A(418-432aa AYKSSQLQMGQKKNS), different from the mouse sequence by one amino acid.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection System
We provide a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit (please inquire) in WB, supported by (please inquire) in IHC-p.
Preparation and Storage
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(Anti-5HT2A Receptor antibody, AAA11510, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-5HT2A Receptor antibody, AAA11510, IHC(P)IHC(P): Rat Brain Tissue)
WB (Western Blot)
(Anti-5HT2A Receptor antibody, AAA11510, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue LysateLane 5: U87 Cell LysateLane 6: SMMC Cell LysateLane 7: HT1080 Cell LysateLane 8: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-5HT2A Receptor antibody, AAA11510, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue LysateLane 5: U87 Cell LysateLane 6: SMMC Cell LysateLane 7: HT1080 Cell LysateLane 8: COLO320 Cell Lysate)
Related Product Information for anti-HTR2A antibody
Background: The mammalian HTR2A(5-HT2A receptor) is a subtype of the 5-HT2 receptor that belongs to the serotonin receptor family and is a G protein-coupled receptor(GPCR). This is the main excitatory receptor subtype among the GPCRs for serotonin(5-HT), although 5-HT2A may also have an inhibitory effect on certain areas such as the visual cortex and the orbit frontal cortex. This receptor was given importance first as the target of psychedelic drugs like LSD. Later it came back to prominence because it was also found to be mediating, at least partly, the action of many antipsychotic drugs, especially the atypical ones.5-HT2A also happens to be a necessary receptor for the spread of the human polyoma virus called JC virus. Sparkes et al.(1991) concluded that the gene is located on 13q14-q21 in man and on chromosome 14 in the mouse.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
5-hydroxytryptamine receptor 2A
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled
NCBI Official Symbol
HTR2A
NCBI Official Synonym Symbols
HTR2; 5-HT2A
NCBI Protein Information
5-hydroxytryptamine receptor 2A; 5-HT-2A; 5-HT2 receptor; serotonin 5-HT-2A receptor
UniProt Protein Name
5-hydroxytryptamine receptor 2A
UniProt Gene Name
HTR2A
UniProt Synonym Gene Names
HTR2; 5-HT-2; 5-HT-2A
UniProt Entry Name
5HT2A_HUMAN
Similar Products
Product Notes
The HTR2A htr2a (Catalog #AAA11510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-5HT2A Receptor antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 5HT2A Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot (WB): Concentration: 0.1-0.5ug/ml Tested Species: Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Mouse, Rat Predicted Species: Human Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the HTR2A htr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5HT2A Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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