IHC (Immunohistochemisry)
(Immunofluorescent analysis of HepG2 cells using AAA232883 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human, Mouse AAK1 Polyclonal Antibody | anti-AAK1 antibody
AAK1 Antibody
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
AAK1, Antibody; AAK1 Antibody; AP2-associated protein kinase 1; Adaptor-associated kinase 1; AAK1; KIAA1048; anti-AAK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
961
Applicable Applications for anti-AAK1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human AP2-associated protein kinase 1 protein (274-465AA)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-130105 / sc-134662
Immunogen Species
Homo sapiens (Human)
Research Area
Signal Transduction
Target Names
AAK1
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HepG2 cells using AAA232883 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HepG2 cells using AAA232883 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human brain tissue using AAA232883 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human brain tissue using AAA232883 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in:Mouse kidney tissueAll lanes: AAK1 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 104,94 kDaObserved band size: 104 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:Mouse kidney tissueAll lanes: AAK1 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 104,94 kDaObserved band size: 104 kDa)
Related Product Information for anti-AAK1 antibody
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
AP2-associated protein kinase 1
NCBI Official Synonym Full Names
AP2 associated kinase 1
NCBI Official Symbol
AAK1
NCBI Protein Information
AP2-associated protein kinase 1
UniProt Protein Name
AP2-associated protein kinase 1
UniProt Gene Name
AAK1
UniProt Synonym Gene Names
KIAA1048
UniProt Entry Name
AAK1_HUMAN
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Product Notes
The AAK1 aak1 (Catalog #AAA232883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AAK1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AAK1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the AAK1 aak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AAK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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