FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ABCC3 antibody (AAA127116).Overlay histogram showing HepG2 cells stained with AAA127116 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCC3 Antibody (AAA127116, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human ABCC3 Polyclonal Antibody | anti-ABCC3 antibody
Anti-ABCC3 Antibody Picoband
Gene Names
ABCC3; MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
ABCC3, Antibody; Anti-ABCC3 Antibody Picoband; BAG family molecular chaperone regulator 1; Fc fragment of IgG receptor Ia; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD64; FCGR1A; FCG1; FCGR1; IGFR1; anti-ABCC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Monocyte/macrophage specific.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-ABCC3 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
E coli-derived human ABCC3 recombinant protein (Position: H22-G1351).
Subcellular Localization
Cell membrane
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ABCC3 antibody (AAA127116).Overlay histogram showing HepG2 cells stained with AAA127116 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCC3 Antibody (AAA127116, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ABCC3 antibody (AAA127116).Overlay histogram showing HepG2 cells stained with AAA127116 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCC3 Antibody (AAA127116, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of ABCC3 using anti-ABCC3 antibody (AAA127116).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC3 antigen affinity purified polyclonal antibody (#AAA127116) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCC3 at approximately 200 kDa. The expected band size for ABCC3 is at 200 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of ABCC3 using anti-ABCC3 antibody (AAA127116).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC3 antigen affinity purified polyclonal antibody (#AAA127116) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCC3 at approximately 200 kDa. The expected band size for ABCC3 is at 200 kDa.)
Related Product Information for anti-ABCC3 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-ABCC3 antibody
References
1. Belinsky, M. G., Bain, L. J., Balsara, B. B., Testa, J. R., Kruh, G. D. Characterization of MOAT-C and MOAT-D, new members of the MRP/cMOAT subfamily of transporter proteins. J. Nat. Cancer Inst. 90: 1735-1741, 1998. 2. Fromm, M. F., Leake, B., Roden, D. M., Wilkinson, G. R., Kim, R. B. Human MRP3 transporter: identification of the 5-prime flanking region, genomic organization and alternative splice variants. Biochim. Biophys. Acta 1415: 369-374, 1999. 3. Kiuchi, Y., Suzuki, H., Hirohashi, T., Tyson, C. A., Sugiyama, Y. cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3). FEBS Lett. 433: 149-152,1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
169,343 Da
NCBI Official Full Name
canalicular multispecific organic anion transporter 2 isoform 2
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family C (CFTR/MRP), member 3
NCBI Official Symbol
ABCC3
NCBI Official Synonym Symbols
MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757
NCBI Protein Information
canalicular multispecific organic anion transporter 2; multidrug resistance associated protein; multidrug resistance-associated protein 3; ATP-binding cassette sub-family C member 3; multi-specific organic anion transporter D; canicular multispecific orga
UniProt Protein Name
Canalicular multispecific organic anion transporter 2
UniProt Gene Name
ABCC3
UniProt Synonym Gene Names
CMOAT2; MLP2; MRP3; MOAT-D
UniProt Entry Name
MRP3_HUMAN
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Product Notes
The ABCC3 abcc3 (Catalog #AAA127116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ABCC3 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ABCC3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ABCC3 abcc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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