Rabbit anti-Human ABCC5 Polyclonal Antibody | anti-ABCC5 antibody

ABCC5 Antibody (Center)

Cat. Num. AAA284077

• Gene Names: ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ABCC5, Antibody; ABCC5 Antibody (Center); Multidrug resistance-associated protein 5; ATP-binding cassette sub-family C member 5; Multi-specific organic anion transporter C; MOAT-C; SMRP; pABC11; ABCC5; MRP5; anti-ABCC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This ABCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 625-652 amino acids from the Central region of human ABCC5.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Applicable Applications for anti-ABCC5 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Region: 625-652
• Function: ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro) (PubMed:10893247, PubMed:12637526, PubMed:12695538, PubMed:15899835, PubMed:17229149, PubMed:25964343). Acts also as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins (PubMed:26515061). Confers resistance to the antiviral agent PMEA (PubMed:12695538). Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated (PubMed:10840050, PubMed:12435799, PubMed:12695538, PubMed:15899835). Acts as a heme transporter required for the translocation of cytosolic heme to the secretory pathway (PubMed:24836561). May play a role in energy metabolism by regulating the glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells (By similarity).
• Cellular Location: Basolateral cell membrane; Multi-pass membrane protein. Golgi apparatus lumen Endosome membrane. Cytoplasmic granule {ECO:0000250|UniProtKB:Q9R1X5}. Apical cell membrane; Multi-pass membrane protein. Note=In most cells, routes to the basolateral plasma membrane, but in the brain capillary endothelial cells that form the blood-brain barrier, resides in the apical membrane
• Tissue Location: [Isoform 3]: Predominant isoform in retinal pigment epithelium, bladder, and stomach.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of 293 cells using ABCC5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human brain tissue with ABCC5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of ABCC5 Antibody (Center) (AAA284077) in 293 cell line lysates (35ug/lane). ABCC5 (arrow) was detected using the purified Pab.(2ug/ml))

Related Product Information for anti-ABCC5 antibody

ABCC5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs,6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients.

Product Categories/Family for anti-ABCC5 antibody

Signal Transduction

References

Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)

NCBI and Uniprot Product Information

• NCBI GI #: 66529093
• NCBI GeneID: 10057
• NCBI Accession #: NP_001018881.1; NP_005679.2
• NCBI GenBank Nucleotide #: NM_001023587.1
• UniProt Accession #: O15440; O14517; Q29ZA9; Q29ZB1; Q86UX3; Q86W30; Q9UN85; Q9UNP5; Q9UQC3; B9EIQ2
• Molecular Weight: 160660
• NCBI Official Full Name: multidrug resistance-associated protein 5 isoform 2
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• NCBI Official Symbol: ABCC5
• NCBI Official Synonym Symbols: MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• NCBI Protein Information: multidrug resistance-associated protein 5
• UniProt Protein Name: Multidrug resistance-associated protein 5
• UniProt Gene Name: ABCC5
• UniProt Synonym Gene Names: MRP5; MOAT-C
• UniProt Entry Name: MRP5_HUMAN