WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using ABCD4 antibody.The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse ABCD4 Polyclonal Antibody | anti-ABCD4 antibody
ABCD4 Antibody
Gene Names
ABCD4; P70R; P79R; ABC41; MAHCJ; PMP69; PXMP1L; EST352188
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ABCD4, Antibody; ABCD4 Antibody; 69 kDa peroxisomal ABC transporter; ABC 41; ABC41; ABCD 4; ABCD4; ABCD4_HUMAN; ATP binding cassette sub family D (ALD) member 4; ATP binding cassette sub family D member 4; ATP-binding cassette sub-family D member 4; EST352188; MAHCJ; MGC105956; P70R; P79R; Peroxisomal membrane protein 1 like; Peroxisomal membrane protein 1-like; Peroxisomal membrane protein 69; PMP 69; PMP69; PMP70-related protein; PXMP 1L; PXMP1 L; PXMP1-L; PXMP1L; anti-ABCD4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ABCD4 antibody detects endogenous levels of total ABCD4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
606
Applicable Applications for anti-ABCD4 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Peroxisome Membrane.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using ABCD4 antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using ABCD4 antibody.The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA324490 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA324490 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemistry)
(AAA324490 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA324490 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ABCD4 antibody
Function: May be involved in intracellular processing of vitamin B12 (cobalamin). Could play a role in the lysosomal release of vitamin B12 into the cytoplasm.
Subunit Structure: Homodimer or heterodimer.
Similarity: Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Subunit Structure: Homodimer or heterodimer.
Similarity: Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70 kD
Predicted: 69 kDa
Predicted: 69 kDa
NCBI Official Full Name
ATP-binding cassette sub-family D member 4 isoform a
NCBI Official Synonym Full Names
ATP binding cassette subfamily D member 4
NCBI Official Symbol
ABCD4
NCBI Official Synonym Symbols
P70R; P79R; ABC41; MAHCJ; PMP69; PXMP1L; EST352188
NCBI Protein Information
ATP-binding cassette sub-family D member 4
UniProt Protein Name
ATP-binding cassette sub-family D member 4
UniProt Gene Name
ABCD4
UniProt Synonym Gene Names
PXMP1L; P70R; PXMP1-L; PMP69
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Product Notes
The ABCD4 abcd4 (Catalog #AAA324490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABCD4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ABCD4 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ABCD4 abcd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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