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product-image-AAA284770_IF10.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of ABCG1 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Rabbit anti-Human, mouse ABCG1 Polyclonal Antibody | anti-ABCG1 antibody

ABCG1 Antibody (N-term)

Gene Names
ABCG1; ABC8; WHITE1
Reactivity
Human, mouse
Applications
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ABCG1, Antibody; ABCG1 Antibody (N-term); ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog; ABCG1; ABC8; WHT1; anti-ABCG1 antibody
Ordering
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the N-terminal region of human ABCG1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
168-195
Sequence Length
785
Applicable Applications for anti-ABCG1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of ABCG1 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

product-image-AAA284770_IF10.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of ABCG1 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemisry)

(ABCG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA284770_IHC11.jpg IHC (Immunohistochemisry) (ABCG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

FCM/FACS (Flow Cytometry)

(ABCG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA284770_FCM13.jpg FCM/FACS (Flow Cytometry) (ABCG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of ABCG1 antibody (N-term) in mouse spleen tissue lysates (35ug/lane). ABCG1 (arrow) was detected using the purified Pab.)

product-image-AAA284770_WB15.jpg WB (Western Blot) (Western blot analysis of ABCG1 antibody (N-term) in mouse spleen tissue lysates (35ug/lane). ABCG1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ABCG1 antibody
ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.
Product Categories/Family for anti-ABCG1 antibody
References
Furuyama,S., J. Atheroscler. Thromb. 16 (3), 194-200 (2009)
Stefulj,J., Circ. Res. 104 (5), 600-608 (2009)
Mauerer,R., Exp. Mol. Med. 41 (2), 126-132 (2009)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
75592
NCBI Official Full Name
ATP-binding cassette sub-family G member 1 isoform 4
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family G (WHITE), member 1
NCBI Official Symbol
ABCG1
NCBI Official Synonym Symbols
ABC8; WHITE1
NCBI Protein Information
ATP-binding cassette sub-family G member 1
UniProt Protein Name
ATP-binding cassette sub-family G member 1
UniProt Gene Name
ABCG1
UniProt Synonym Gene Names
ABC8; WHT1
UniProt Entry Name
ABCG1_HUMAN

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Product Notes

The ABCG1 abcg1 (Catalog #AAA284770) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 168-195. The ABCG1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ABCG1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ABCG1 abcg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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