Rabbit ABL1/2 Polyclonal Antibody | anti-ABL1/2 antibody

Phospho-ABL1/2 (Tyr70/Tyr116) Antibody

Cat. Num. AAA329705

• Gene Names: ABL1; ABL; JTK7; p150; c-ABL; v-abl; bcr/abl
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (100%)
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: ABL1/2, Antibody; Phospho-ABL1/2 (Tyr70/Tyr116) Antibody; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine protein kinase 1; Abl 1; ABL; ABL proto oncogene 1 non receptor tyrosine kinase; ABL1; ABL1_HUMAN; bcr/abl; bcr/c abl oncogene protein; c ABL; c abl oncogene 1 non receptor tyrosine kinase; c abl oncogene 1 receptor tyrosine kinase; c ABL1; JTK7; p150; Proto oncogene tyrosine protein kinase ABL1; Proto-oncogene c-Abl; Tyrosine-protein kinase ABL1; v abl Abelson murine leukemia viral oncogene homolog 1; v abl; Abelson murine leukemia viral oncogene homolog 2; Abelson related gene protein; Abelson tyrosine-protein kinase 2; Abelson-related gene protein; ABL2; ABL2_HUMAN; ABLL; ARG; Tyrosine kinase ARG; Tyrosine protein kinase ABL2; Tyrosine-protein kinase ARG; v abl Abelson murine leukemia viral oncogene homolog 2; anti-ABL1/2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-ABL1/2 (Tyr70/Tyr116) Antibody detects endogenous levels of ABL1/2 onlyPhospho-ABL1/2 (Tyr70/Tyr116) Antibody detects endogenous levels of ABL1 when phosphorylated at Tyr70 or ABL2 when phosphorylated at Tyr116.; Tissue Specificity: Widely expressed.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ABL1/2 antibody: ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human ABL1/2 around the phosphorylation site of Tyr70/Tyr116.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
• Subunit Structure: Interacts with PSMA7. Interacts with CTTN. Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases.
• Similarity: Contains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments (By similarity).Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
• Subcellular Location: Cytoplasm>Cytoskeleton.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(Staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of ABL1/2 (Phospho-Tyr70/Tyr116) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-ABL1/2 antibody

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

NCBI and Uniprot Product Information

• NCBI GI #: 62362414
• NCBI GeneID: 25
• NCBI Accession #: NP_005148.2
• NCBI GenBank Nucleotide #: NM_005157.4
• UniProt Accession #: P00519; P42684
• Molecular Weight: 122,873 Da
• NCBI Official Full Name: tyrosine-protein kinase ABL1 isoform a
• NCBI Official Synonym Full Names: c-abl oncogene 1, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL1
• NCBI Official Synonym Symbols: ABL; JTK7; p150; c-ABL; v-abl; bcr/abl
• NCBI Protein Information: tyrosine-protein kinase ABL1; proto-oncogene c-Abl; bcr/c-abl oncogene protein; Abelson tyrosine-protein kinase 1; c-abl oncogene 1, receptor tyrosine kinase; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolo
• UniProt Protein Name: Tyrosine-protein kinase ABL1
• UniProt Gene Name: ABL1
• UniProt Synonym Gene Names: ABL; JTK7
• UniProt Entry Name: ABL1_HUMAN