IP (Immunoprecipitation)
(Detection of human Abraxas by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Abraxas antibody AAA211794 used for IP at 10 ug/mg lysate. Abraxas was also immunoprecipitated by rabbit anti-Abraxas antibody which recognizes an upstream epitope. For blotting immunoprecipitated Abraxas, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human Abraxas Polyclonal Antibody | anti-Abraxas antibody
Rabbit anti-Abraxas Antibody, Affinity Purified
Gene Names
FAM175A; ABRA1; CCDC98
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Abraxas, Antibody; Rabbit anti-Abraxas Antibody, Affinity Purified; coiled-coil domain containing 98; family with sequence similarity 175 member A; Protein FAM175A; FAM175A; Coiled-coil domain-containing protein 98; CCDC98; BRCA1-A complex subunit Abraxas 1; BRCA1-A complex subunit Abraxas; ABRA1; abraxas protein; abraxas 1, BRCA1 A complex subunit; anti-Abraxas antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Abraxas antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 359 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Abraxas by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Abraxas antibody AAA211794 used for IP at 10 ug/mg lysate. Abraxas was also immunoprecipitated by rabbit anti-Abraxas antibody which recognizes an upstream epitope. For blotting immunoprecipitated Abraxas, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Abraxas by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Abraxas antibody AAA211794 used for IP at 10 ug/mg lysate. Abraxas was also immunoprecipitated by rabbit anti-Abraxas antibody which recognizes an upstream epitope. For blotting immunoprecipitated Abraxas, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Abraxas by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Abraxas (Cat. No. AAA211794) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Abraxas by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Abraxas (Cat. No. AAA211794) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Abraxas antibody
Abraxas was identified in a phosphopeptide affinity proteomic and mass spec analysis as a protein that bound directly to the BRCA1 BRCT repeat. Along with RAP80, Abraxas was required to form a complex with BRCA1 for the DNA damage response.
Product Categories/Family for anti-Abraxas antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,663 Da
NCBI Official Full Name
BRCA1-A complex subunit Abraxas
NCBI Official Synonym Full Names
family with sequence similarity 175, member A
NCBI Official Symbol
FAM175A
NCBI Official Synonym Symbols
ABRA1; CCDC98
NCBI Protein Information
BRCA1-A complex subunit Abraxas; abraxas protein; coiled-coil domain containing 98; coiled-coil domain-containing protein 98
UniProt Protein Name
BRCA1-A complex subunit Abraxas
UniProt Gene Name
FAM175A
UniProt Synonym Gene Names
ABRA1; CCDC98
UniProt Entry Name
F175A_HUMAN
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Product Notes
The Abraxas fam175a (Catalog #AAA211794) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Abraxas Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Abraxas can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Abraxas fam175a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Abraxas, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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