ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CSB-PA010403OA120acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
Rabbit anti-Human Acetyl-Histone H2B Polyclonal Antibody | anti-HIST1H2BC antibody
Acetyl-Histone H2B (K120) Antibody
Gene Names
HIST1H2BG; H2B/a; H2BFA; H2B.1A; dJ221C16.8
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Antigen Affinity Purified
Synonyms
Acetyl-Histone H2B, Antibody; Acetyl-Histone H2B (K120) Antibody; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l; HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI; H2BFL; H2BFH; H2BFG; H2BFA; H2BFK; anti-HIST1H2BC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
126
Applicable Applications for anti-HIST1H2BC antibody
ChIP (Chromatin immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA
Immunogen Species
Human
Immunogen
Peptide sequence around site of acetyl-Lys (120) derived from Human Histone H2B type 1-C/E/F/G/I
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CSB-PA010403OA120acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CSB-PA010403OA120acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA120acHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA120acHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of CSB-PA010403OA120acHU diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of CSB-PA010403OA120acHU diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Product Categories/Family for anti-HIST1H2BC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,906 Da
NCBI Official Full Name
histone H2B type 1-C/E/F/G/I
NCBI Official Synonym Full Names
histone cluster 1 H2B family member g
NCBI Official Symbol
HIST1H2BG
NCBI Official Synonym Symbols
H2B/a; H2BFA; H2B.1A; dJ221C16.8
NCBI Protein Information
histone H2B type 1-C/E/F/G/I
UniProt Protein Name
Histone H2B type 1-C/E/F/G/I
UniProt Gene Name
HIST1H2BC
UniProt Synonym Gene Names
H2BFL; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l
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Product Notes
The HIST1H2BC hist1h2bc (Catalog #AAA235317) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetyl-Histone H2B (K120) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Acetyl-Histone H2B can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA. Researchers should empirically determine the suitability of the HIST1H2BC hist1h2bc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetyl-Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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