WB (Western Blot)
(Detection of human ACF1/BAZ1A by western blot. Samples: Whole cell lysate (25 ug) from K-562, HEK293T, MCF-7, RKO, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACF1/BAZ1A antibody (AAA211416 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human ACF1/BAZ1A Polyclonal Antibody | anti-ACF1/BAZ1A antibody
Rabbit anti-ACF1/BAZ1A Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ACF1/BAZ1A, Antibody; Rabbit anti-ACF1/BAZ1A Antibody, Affinity Purified; WALp1; CHRAC subunit ACF1; WCRF180; hWALp1; hACF1; ATP-utilizing chromatin assembly and remodeling factor 1; ATP-dependent chromatin-remodeling protein; ACF1; ATP-dependent chromatin remodeling protein; williams syndrome transcription factor-related chromatin-remodeling factor 180; bromodomain adjacent to zinc finger domain protein 1A; Bromodomain adjacent to zinc finger domain protein 1A; bromodomain adjacent to zinc finger domain 1A; anti-ACF1/BAZ1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ACF1/BAZ1A antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 725 and 775
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ACF1/BAZ1A by western blot. Samples: Whole cell lysate (25 ug) from K-562, HEK293T, MCF-7, RKO, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACF1/BAZ1A antibody (AAA211416 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human ACF1/BAZ1A by western blot. Samples: Whole cell lysate (25 ug) from K-562, HEK293T, MCF-7, RKO, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACF1/BAZ1A antibody (AAA211416 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ACF1/BAZ1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACF1/BAZ1A antibody (AAA211416 lot 2) used for IP at 6 ug per reaction. ACF1/BAZ1A was also immunoprecipitated by a previous lot of this antibody (AAA211416 lot 1) and a second antibody against a different epitope of ACF1/BAZ1A (BL5377). For blotting immunoprecipitated ACF1/BAZ1A, AAA211416 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ACF1/BAZ1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACF1/BAZ1A antibody (AAA211416 lot 2) used for IP at 6 ug per reaction. ACF1/BAZ1A was also immunoprecipitated by a previous lot of this antibody (AAA211416 lot 1) and a second antibody against a different epitope of ACF1/BAZ1A (BL5377). For blotting immunoprecipitated ACF1/BAZ1A, AAA211416 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-ACF1/BAZ1A antibody
ACF1/BAZ1A is a subunit of the ACF chromatin-remodeling complex and the ISWI CHRAC remodeling complex. In the ACF complex, ACF1/BAZ1A functions to influence the nucleosome remodeling activity of the SNF2h ATPase. As part of the CHRAC complex, ACF1/BAZ1A may function to target CHRAC to heterochromatin. ACF1 has also been shown to interact with the nuclear receptor corepressor protein (N-CoR) to facilitate the repression of transcription by unliganded nuclear receptors.
Product Categories/Family for anti-ACF1/BAZ1A antibody
NCBI and Uniprot Product Information
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Product Notes
The ACF1/BAZ1A baz1a (Catalog #AAA211416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ACF1/BAZ1A Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACF1/BAZ1A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ACF1/BAZ1A baz1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACF1/BAZ1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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