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FCM/FACS (Flow Cytometry)
(ACTA1/alpha-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ACTA1/alpha-actin Polyclonal Antibody | anti-ACTA1 antibody
ACTA1/alpha-actin Antibody (C-term)
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
HumanPredicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
ACTA1/alpha-actin, Antibody; ACTA1/alpha-actin Antibody (C-term); ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; anti-ACTA1 antibody
Host
Rabbit
Reactivity
Human
Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
377
Applicable Applications for anti-ACTA1 antibody
ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Antigen Source
Human
Antigen Region
346-375
Antigen Type
Synthetic Peptide
Function
Actins are highly conserved proteins that areinvolved in various types of cell motility andare ubiquitously expressed in all eukaryotic cells.
Cellular Location
Cytoplasm, cytoskeleton.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ACTA1/alpha-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ACTA1/alpha-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ACTA1/alpha-actin Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ACTA1/alpha-actin Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(ACTA1/alpha-actin Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/alpha-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ACTA1/alpha-actin Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/alpha-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from human placenta tissue lysate,RD cell line (from left to right), using ACTA1/alpha-actin Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysates from human placenta tissue lysate,RD cell line (from left to right), using ACTA1/alpha-actin Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Related Product Information for anti-ACTA1 antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Product Categories/Family for anti-ACTA1 antibody
References
Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010). Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010). Yu, G., et al. J Clin Neurosci 17(6):766-769(2010). Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010). Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW:
H=42
M=42
Rat=42 KDa
H=42
M=42
Rat=42 KDa
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA290945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTA1/alpha-actin Antibody (C-term) reacts with Human Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's ACTA1/alpha-actin can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTA1/alpha-actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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