IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit Actin (ACTB/ACTC) Polyclonal Antibody | anti-ACTC1 antibody
Actin (ACTB/ACTC) Antibody (N-term)
Gene Names
ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4
Reactivity
Human, mouse (Predicted Reactivity: C Elegans, Drosophila, Xenopus, Chicken, Bovine, Rabbit, Rat, Zebrafish, Hamster, Monkey, Pig, Yeast)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Actin (ACTB/ACTC), Antibody; Actin (ACTB/ACTC) Antibody (N-term); Actin; alpha cardiac muscle 1; Alpha-cardiac actin; ACTC1; ACTC; anti-ACTC1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: C Elegans, Drosophila, Xenopus, Chicken, Bovine, Rabbit, Rat, Zebrafish, Hamster, Monkey, Pig, Yeast)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human Actin (ACTB/ACTC).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
38-67
Sequence Length
377
Applicable Applications for anti-ACTC1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab.)
Related Product Information for anti-ACTC1 antibody
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
Product Categories/Family for anti-ACTC1 antibody
References
Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007) Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007) Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42019
NCBI Official Full Name
actin, alpha cardiac muscle 1 proprotein
NCBI Official Synonym Full Names
actin, alpha, cardiac muscle 1
NCBI Official Symbol
ACTC1
NCBI Official Synonym Symbols
ACTC; ASD5; CMD1R; CMH11; LVNC4
NCBI Protein Information
actin, alpha cardiac muscle 1
UniProt Protein Name
Actin, alpha cardiac muscle 1
UniProt Gene Name
ACTC1
UniProt Synonym Gene Names
ACTC
UniProt Entry Name
ACTC_HUMAN
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Product Notes
The ACTC1 actc1 (Catalog #AAA289050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 38-67. The Actin (ACTB/ACTC) Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: C Elegans, Drosophila, Xenopus, Chicken, Bovine, Rabbit, Rat, Zebrafish, Hamster, Monkey, Pig, Yeast) and may cross-react with other species as described in the data sheet. AAA Biotech's Actin (ACTB/ACTC) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ACTC1 actc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin (ACTB/ACTC), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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