Application Data
Rabbit Actin (N-terminal Region) Polyclonal Antibody
Actin (N-terminal Region)
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Actin (N-terminal Region), Antibody; Actin (N-terminal Region); beta-Actin; anti-Actin (N-terminal Region) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Polyclonal
Specificity
This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of human A431, SYF, and HUVEC cells, as well as mouse C2C12 cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Actin (N-terminal Region) antibody
WB (Western Blot), ELISA
Immunogen
Actin synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human b actin. This sequence is identical to similar regions in all four a actins, as well as in g actin, and is well conserved in actins from most eukaryotic species.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Application Data
Application Data
Application Data
Application Data
Related Product Information for anti-Actin (N-terminal Region) antibody
Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four a isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (b and g). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
References
• Baba, T. et al. (2003) J. Immunol. 170: 3762.
• Jungbluth, A. et al. (1995) FEBS Let. 375:87.
• Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
• Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694
• Jungbluth, A. et al. (1995) FEBS Let. 375:87.
• Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
• Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694
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Product Notes
The Actin (N-terminal Region) (Catalog #AAA71510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin (N-terminal Region) reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Actin (N-terminal Region) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the Actin (N-terminal Region) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin (N-terminal Region), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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