IF (Immunofluorescence)
(AAA327785 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA327785) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
Rabbit anti-Human, Mouse ACTL6B Polyclonal Antibody | anti-ACTL6B antibody
ACTL6B Antibody
Gene Names
ACTL6B; ACTL6; BAF53B; arpNalpha
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Peptide affinity purification
Synonyms
ACTL6B, Antibody; ACTL6B Antibody; 53 kDa BRG1 associated factor B; 53 kDa BRG1-associated factor B; ACL 6B; ACL6B; ACL6B_HUMAN; Actin like 6; Actin like protein 6B; Actin like protein6B; Actin related protein; Actin related protein Baf53b; actin-like 6B; Actin-like protein 6B; Actin-related protein Baf53b; Actl 6; ACTL 6B; Actl6; ACTL6 B; Actl6b; Arp Na; ArpN a; ArpN alpha; ArpNa; ArpNalpha; Baf 53b; BAF53B; BRG1-associated factor 53B; anti-ACTL6B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
ACTL6B Antibody detects endogenous levels of total ACTL6B.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ACTL6B antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human ACTL6B,corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA327785 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA327785) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(AAA327785 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA327785) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of ACTL6B using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of ACTL6B using HeLa whole cell lysates)
Related Product Information for anti-ACTL6B antibody
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex), as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47 kDa
NCBI Official Full Name
actin-like protein 6B
NCBI Official Synonym Full Names
actin like 6B
NCBI Official Symbol
ACTL6B
NCBI Official Synonym Symbols
ACTL6; BAF53B; arpNalpha
NCBI Protein Information
actin-like protein 6B
UniProt Protein Name
Actin-like protein 6B
UniProt Gene Name
ACTL6B
UniProt Synonym Gene Names
ACTL6; BAF53B; BAF53B
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Product Notes
The ACTL6B actl6b (Catalog #AAA327785) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTL6B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACTL6B can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ACTL6B actl6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTL6B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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