Application Data
(Sample:Heart (Mouse) Lysate at 40 ug. Primary: Anti-ADAM12 at 1/500 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 77/100 kD. Observed band size: 115 kD.)
Rabbit ADAM12 Polyclonal Antibody | anti-ADAM12 antibody
ADAM12
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
Applications
ELISA, Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot
Purity
Protein A affinity purified.
Synonyms
ADAM12, Antibody; ADAM12; A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; Meltrin alpha; Meltrin-alpha; MLTN; MLTNA; OTTHUMP00000046766; anti-ADAM12 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A affinity purified.
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-ADAM12 antibody
ELISA, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from human ADAM12 201-300/909
Conjugation
AF750
Positive control
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Subcellular location
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Isoform
Isoform3: Secreted (Potential).
Isoform 4: Secreted (Potential).
Isoform 4: Secreted (Potential).
Preparation and Storage
Store at -20? for one year. Avoid repeated freeze/thaw cycles.
Application Data
(Sample:Heart (Mouse) Lysate at 40 ug. Primary: Anti-ADAM12 at 1/500 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 77/100 kD. Observed band size: 115 kD.)
Application Data
(Sample:Heart (Mouse) Lysate at 40 ug. Primary: Anti-ADAM12 at 1/500 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 77/100 kD. Observed band size: 115 kD.)
Application Data
(Blank control (Black line): U87MG (Black).Primary Antibody (green line): Rabbit Anti-ADAM12 antibody. Dilution: 1 ug /106 cells; Isotype Control Antibody (orange line): Rabbit IgG.Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE. Dilution: 1ug/test. Protocol: The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Application Data
(Blank control (Black line): U87MG (Black).Primary Antibody (green line): Rabbit Anti-ADAM12 antibody. Dilution: 1 ug /106 cells; Isotype Control Antibody (orange line): Rabbit IgG.Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE. Dilution: 1ug/test. Protocol: The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Related Product Information for anti-ADAM12 antibody
Description: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
77/100 KDa
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Product Notes
The ADAM12 (Catalog #AAA268969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAM12 reacts with Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's ADAM12 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the ADAM12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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