IF (Immunofluorescence)
(MCF-7 cells were stained using 5% goat serum, followed by counterstaining with DAPI. Cells were fixed in 4% formaldehyde and blocked with 10% normal goat serum. After blocking, cells were incubated overnight at 4°C with the primary antibody. The secondary antibody used was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human ADAM8 Polyclonal Antibody | anti-ADAM8 antibody
ADAM8 Antibody
IF (Immunofluorescence)
(MCF-7 cells were stained using 5% goat serum, followed by counterstaining with DAPI. Cells were fixed in 4% formaldehyde and blocked with 10% normal goat serum. After blocking, cells were incubated overnight at 4°C with the primary antibody. The secondary antibody used was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(MCF-7 cells were stained using 5% goat serum, followed by counterstaining with DAPI. Cells were fixed in 4% formaldehyde and blocked with 10% normal goat serum. After blocking, cells were incubated overnight at 4°C with the primary antibody. The secondary antibody used was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF-7 cell with AAA234616 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF-7 cell with AAA234616 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(HeLa cells were stained using the primary antibody AAA234616 at a dilution of 1:30, followed by counterstaining with DAPI.Cells were fixed in 4% formaldehyde and blocked with 10% normal goat serum.After blocking, cells were incubated overnight at 4°C with the primary antibody.The secondary antibody used was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(HeLa cells were stained using the primary antibody AAA234616 at a dilution of 1:30, followed by counterstaining with DAPI.Cells were fixed in 4% formaldehyde and blocked with 10% normal goat serum.After blocking, cells were incubated overnight at 4°C with the primary antibody.The secondary antibody used was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
WB (Western Blot)
(Positive WB detected in: THP-1 whole cell lysate(20ug), K562 whole cell lysate(20ug), JK whole cell lysate(20ug)All lanes: ADAM8 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 79,81 kDaObserved band size: 100 kDaExposure time: 300s)
WB (Western Blot)
(Positive WB detected in: THP-1 whole cell lysate(20ug), K562 whole cell lysate(20ug), JK whole cell lysate(20ug)All lanes: ADAM8 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 79,81 kDaObserved band size: 100 kDaExposure time: 300s)
NCBI and Uniprot Product Information
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Product Notes
The ADAM8 adam8 (Catalog #AAA234616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAM8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADAM8 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ADAM8 adam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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