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FCM/FACS (Flow Cytometry)
(ADCYAP1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ADCYAP1 Polyclonal Antibody | anti-ADCYAP1 antibody
ADCYAP1 Antibody (C-term)
Gene Names
ADCYAP1; PACAP
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ADCYAP1, Antibody; ADCYAP1 Antibody (C-term); Pituitary adenylate cyclase-activating polypeptide; PACAP; PACAP-related peptide; PRP-48; Pituitary adenylate cyclase-activating polypeptide 27; PACAP-27; PACAP27; Pituitary adenylate cyclase-activating polypeptide 38; PACAP-38; PACAP38; ADCYAP1; anti-ADCYAP1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ADCYAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the C-terminal region of human ADCYAP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.44 (varies by lot)
Sequence Positions
148-176
Sequence Length
176
Applicable Applications for anti-ADCYAP1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ADCYAP1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ADCYAP1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ADCYAP1 Antibody (C-term) with 293 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ADCYAP1 Antibody (C-term) with 293 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(ADCYAP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADCYAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ADCYAP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADCYAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ADCYAP1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ADCYAP1 antibody detected the ADCYAP1 protein (arrow).)
WB (Western Blot)
(ADCYAP1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ADCYAP1 antibody detected the ADCYAP1 protein (arrow).)
Related Product Information for anti-ADCYAP1 antibody
ADCYAP1 is adenylate cyclase activating polypeptide
1. Mediated by adenylate cyclase activating polypeptide 1
receptors, this polypeptide stimulates adenylate cyclase and
subsequently increases the cAMP level in target cells. Adenylate
cyclase activating polypeptide 1 is not only a hypophysiotropic
hormone, but also functions as a neurotransmitter and
neuromodulator. In addition, it plays a role in paracrine and
autocrine regulation of certain types of cells. This gene encodes
three different mature peptides, including two isotypes, a shorter
form and a longer form.
1. Mediated by adenylate cyclase activating polypeptide 1
receptors, this polypeptide stimulates adenylate cyclase and
subsequently increases the cAMP level in target cells. Adenylate
cyclase activating polypeptide 1 is not only a hypophysiotropic
hormone, but also functions as a neurotransmitter and
neuromodulator. In addition, it plays a role in paracrine and
autocrine regulation of certain types of cells. This gene encodes
three different mature peptides, including two isotypes, a shorter
form and a longer form.
Product Categories/Family for anti-ADCYAP1 antibody
References
Hosoya, M., et al. Biochim. Biophys. Acta 1129(2):199-206(1992)
Ohkubo, S., et al. DNA Cell Biol. 11(1):21-30(1992)
Kimura, C., et al. Biochem. Biophys. Res. Commun. 166(1):81-89(1990)
Ohkubo, S., et al. DNA Cell Biol. 11(1):21-30(1992)
Kimura, C., et al. Biochem. Biophys. Res. Commun. 166(1):81-89(1990)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18835
NCBI Official Full Name
pituitary adenylate cyclase-activating polypeptide preproprotein
NCBI Official Synonym Full Names
adenylate cyclase activating polypeptide 1 (pituitary)
NCBI Official Symbol
ADCYAP1
NCBI Official Synonym Symbols
PACAP
NCBI Protein Information
pituitary adenylate cyclase-activating polypeptide
UniProt Protein Name
Pituitary adenylate cyclase-activating polypeptide
UniProt Gene Name
ADCYAP1
UniProt Synonym Gene Names
PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38
UniProt Entry Name
PACA_HUMAN
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Product Notes
The ADCYAP1 adcyap1 (Catalog #AAA284655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 148-176. The ADCYAP1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ADCYAP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ADCYAP1 adcyap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADCYAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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