WB (Western Blot)
Rabbit Adenine Nucleotide Translocator 2 Polyclonal Antibody | anti-SLC25A5 antibody
Adenine Nucleotide Translocator 2 Antibody
Gene Names
SLC25A5; T2; T3; 2F1; AAC2; ANT2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Synonyms
Adenine Nucleotide Translocator 2, Antibody; Adenine Nucleotide Translocator 2 Antibody; 2F1; AAC2; Adenine nucleotide translocator 2; ADP; ADP ATP carrier protein 2; ANT2; ATP carrier protein 2; fibroblast isoform; SLC25A5; Solute carrier family 25 member 5; T2; T3 antibody; anti-SLC25A5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
One hundred micrograms (100ug) of antibody stabilization buffer.
Concentration
0.75 mg/ml in antibody stabilization buffer (varies by lot)
Sequence Length
298
Applicable Applications for anti-SLC25A5 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, CM (Confocal Microscopy)
Immunogen
Synthetic peptide taken within amino acid region 150-200 on human ADP/ATP translocase 2.
Preparation and Storage
-20°C for long term storage
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-SLC25A5 antibody
Affinity Purified ADP/ATP Translocase 2 Antibody.
Overview: Human Adenine Nucleotide Translocator (ANT) presents four isoforms (ANT1-ANT4), each with a specific expression depending on the nature of the tissue, cell type, developmental stage and status of cell proliferation. ANT1 is specific to muscle and brain tissues, ANT2 occurs mainly in proliferative, undifferentiated cells, ANT3 is ubiquitous, and ANT4 is found in germ cells. ANT2 is a homodimer and a component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. ANT2, also known as solute carrier family 25 mitochondrial carrier member 5 (SLC25A5, is a 298 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and contains three solcar repeats. ANT2 functions as a homodimer that facilitates the exchange of ADP and ATP between the mitochondrion and the cytosol, thus linking the compartment of ATP production (within the mitochondrion) to the areas of ATP utilization (within the cytosol). ANT2 expression in undifferentiated, proliferating cells correlates with the rate of glycolytic metabolism and is an indicator of carcinogenesis. Suppression of ANT2/SLC25A5 expression by specific RNA interference in human breast cancer cells promotes apoptosis and inhibits tumor cell growth which suggest a cytoprotective role of ANT2/SLC25A5. ANT2 functions differently in metabolic and apoptotic pathways and is implicated in the function of the permeability transition pore complex (PTPC), which regulates the release of mitochondrial products that induce apoptosis.
As part of the mitotic spindle-associated MMXD complex, ANT2 plays a role in chromosome segregation, and it is also known to interact with HIV-1 Vpr protein. ANT2 functions in a similar manner to ANT3 but, unlike ANT3, it also plays a role in the formation of the permeability transition pore complex (PTPC), a structure that is important for the regulation of apoptosis. The expression of ANT2 is linked to and is an important indicator of carcinogenesis. Since the expression of ANT2 is closely linked to the rate of glycolytic metabolism and to the mitochondrial bioenergetics of tumors, it should be taken into account for individualizing cancer treatments and for the development of anticancer strategies. The human genome contains several non-transcribed pseudogenes of this gene. The role of ANT2 as an endogenous inhibitor of MMP (Mitochondrial membrane permeabilization) suggests that its selective inhibition could constitute a promising strategy of chemosensitization.
ANT2-selective antibodies were generated against a peptide taken from mouse and human ANT2 protein. The ANT2-selective antibodies are affinity purified on an immobilized antigen based affinity matrix and the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The ANT2-selective antibodies are fully characterized for applications in western blot and ELISA at the recommended dilutions. Antigenic blocking peptides (P-ANT2) and western blot positive control (PC-ANT2) in ready to use SDS-sample buffer are available. Primary antibody ANT2- 201AP labels a ~36 kDa protein in PC-ANT2 samples and several other tissues. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost
Overview: Human Adenine Nucleotide Translocator (ANT) presents four isoforms (ANT1-ANT4), each with a specific expression depending on the nature of the tissue, cell type, developmental stage and status of cell proliferation. ANT1 is specific to muscle and brain tissues, ANT2 occurs mainly in proliferative, undifferentiated cells, ANT3 is ubiquitous, and ANT4 is found in germ cells. ANT2 is a homodimer and a component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. ANT2, also known as solute carrier family 25 mitochondrial carrier member 5 (SLC25A5, is a 298 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and contains three solcar repeats. ANT2 functions as a homodimer that facilitates the exchange of ADP and ATP between the mitochondrion and the cytosol, thus linking the compartment of ATP production (within the mitochondrion) to the areas of ATP utilization (within the cytosol). ANT2 expression in undifferentiated, proliferating cells correlates with the rate of glycolytic metabolism and is an indicator of carcinogenesis. Suppression of ANT2/SLC25A5 expression by specific RNA interference in human breast cancer cells promotes apoptosis and inhibits tumor cell growth which suggest a cytoprotective role of ANT2/SLC25A5. ANT2 functions differently in metabolic and apoptotic pathways and is implicated in the function of the permeability transition pore complex (PTPC), which regulates the release of mitochondrial products that induce apoptosis.
As part of the mitotic spindle-associated MMXD complex, ANT2 plays a role in chromosome segregation, and it is also known to interact with HIV-1 Vpr protein. ANT2 functions in a similar manner to ANT3 but, unlike ANT3, it also plays a role in the formation of the permeability transition pore complex (PTPC), a structure that is important for the regulation of apoptosis. The expression of ANT2 is linked to and is an important indicator of carcinogenesis. Since the expression of ANT2 is closely linked to the rate of glycolytic metabolism and to the mitochondrial bioenergetics of tumors, it should be taken into account for individualizing cancer treatments and for the development of anticancer strategies. The human genome contains several non-transcribed pseudogenes of this gene. The role of ANT2 as an endogenous inhibitor of MMP (Mitochondrial membrane permeabilization) suggests that its selective inhibition could constitute a promising strategy of chemosensitization.
ANT2-selective antibodies were generated against a peptide taken from mouse and human ANT2 protein. The ANT2-selective antibodies are affinity purified on an immobilized antigen based affinity matrix and the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The ANT2-selective antibodies are fully characterized for applications in western blot and ELISA at the recommended dilutions. Antigenic blocking peptides (P-ANT2) and western blot positive control (PC-ANT2) in ready to use SDS-sample buffer are available. Primary antibody ANT2- 201AP labels a ~36 kDa protein in PC-ANT2 samples and several other tissues. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost
Product Categories/Family for anti-SLC25A5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33-36 kDa
NCBI Official Full Name
ADP/ATP translocase 2
NCBI Official Synonym Full Names
solute carrier family 25 member 5
NCBI Official Symbol
SLC25A5
NCBI Official Synonym Symbols
T2; T3; 2F1; AAC2; ANT2
NCBI Protein Information
ADP/ATP translocase 2
UniProt Protein Name
ADP/ATP translocase 2
UniProt Gene Name
SLC25A5
UniProt Synonym Gene Names
ANT2; ANT 2
UniProt Entry Name
ADT2_HUMAN
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Product Notes
The SLC25A5 slc25a5 (Catalog #AAA75808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Adenine Nucleotide Translocator 2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Adenine Nucleotide Translocator 2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, CM (Confocal Microscopy). Researchers should empirically determine the suitability of the SLC25A5 slc25a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenine Nucleotide Translocator 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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