Rabbit ADORA1 / Adenosine A1 Receptor Polyclonal Antibody | anti-ADORA1 antibody

Rabbit Polyclonal to Human ADORA1 / Adenosine A1 Receptor

Cat. Num. AAA51472

• Gene Names: ADORA1; RDC7
• Reactivity: Human, Rat; Predicted Reactivity: Monkey, Guinea pig, Horse, Pig
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: ADORA1 / Adenosine A1 Receptor, Antibody; Rabbit Polyclonal to Human ADORA1 / Adenosine A1 Receptor; Anti-ADORA1 / Adenosine A1 Receptor Antibody (C-Terminus) IHC-plus; A1 adenosine receptor; A1ar; Adenosine A1 receptor; Adenosine receptor A1; ADO-A1 receptor; RDC7; Human ADORA1; Adenosine A1 Receptor; anti-ADORA1 antibody

• Host: Rabbit
• Reactivity: Human, Rat; Predicted Reactivity: Monkey, Guinea pig, Horse, Pig
• Clonality: Polyclonal
• Specificity: Human Adenosine A1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 326

• Applicable Applications for anti-ADORA1 antibody: IHC (Immunohistochemistry)
• Target: ADORA1/Adenosine A1 Receptor
• Immunogen Description: Synthetic 20 amino acid peptide from C-terminus of human Adenosine A1 Receptor. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Orangutan, Marmoset, Elephant, Horse (95%); Rat, Pig, Guinea pig (90%).
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Immunogen Type: Synthetic peptide
• Immunogen: ADORA1 / Adenosine A1 Receptor antibody was raised against synthetic 20 amino acid peptide from C-terminus of human Adenosine A1 Receptor. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Orangutan, Marmoset, Elephant, Horse (95%); Rat, Pig, Guinea pig (90%); Bovine, Hamster, Panda (85%); Mouse, Dog (80%).
• Antigen Modification: C-Terminus
• Preparation and Storage: aliquot and store uniluted at -20°C or below. Can be stored undiluted at -70°C.Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-Adenosine A1 Receptor antibody IHC of rat brain, Purkinje neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-ADORA1 antibody

The A1 Adenosine Receptor is involved in smooth muscle contraction, control of heart rate, sedation, and lipolysis.

Product Categories/Family for anti-ADORA1 antibody

Family: GPCR; Subfamily: Adenosine

NCBI and Uniprot Product Information

• NCBI GI #: 4501947
• NCBI GeneID: 134
• NCBI Accession #: NP_000665.1
• NCBI GenBank Nucleotide #: NM_000674.2
• UniProt Accession #: P30542; Q6FHK3; Q8TAM8; A6NFY5; A6NGP4; A8K1L3; B3KXQ4; D2CGD0
• NCBI Official Full Name: adenosine receptor A1
• NCBI Official Synonym Full Names: adenosine A1 receptor
• NCBI Official Symbol: ADORA1
• NCBI Official Synonym Symbols: RDC7
• NCBI Protein Information: adenosine receptor A1; adenosine A1 receptor variant 1
• UniProt Protein Name: Adenosine receptor A1
• UniProt Gene Name: ADORA1
• UniProt Entry Name: AA1R_HUMAN