FCM/FACS (Flow Cytometry)
(Overlay histogram showing SH-SY5Y cellsstained with AAA289458 (green line). The cells were fixed with 4% paraformaldehyde (10min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA289458, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goatanti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1(1?g/1x10^6 cells) used under the sameconditions. Acquisition of >10, 000 eventswas performed.)
Rabbit anti-Human ADORA2A Polyclonal Antibody | anti-ADORA2A antibody
ADORA2A Antibody (C-term)
Gene Names
ADORA2A; A2aR; RDC8; ADORA2
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
ADORA2A, Antibody; ADORA2A Antibody (C-term); Adenosine receptor A2a; ADORA2A; ADORA2; anti-ADORA2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ADORA2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 347-381 amino acids from the C-terminal region of human ADORA2A.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-ADORA2A antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Function
Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Cellular Location
Cell membrane {ECO:0000250|UniProtKB:P30543}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P30543} Note=Colocalizes with GAS2L2 at neuronal processes {ECO:0000250|UniProtKB:P30543}
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing SH-SY5Y cellsstained with AAA289458 (green line). The cells were fixed with 4% paraformaldehyde (10min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA289458, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goatanti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1(1?g/1x10^6 cells) used under the sameconditions. Acquisition of >10, 000 eventswas performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing SH-SY5Y cellsstained with AAA289458 (green line). The cells were fixed with 4% paraformaldehyde (10min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA289458, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goatanti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1(1?g/1x10^6 cells) used under the sameconditions. Acquisition of >10, 000 eventswas performed.)
IHC (Immunohiostchemistry)
(AAA289458 staining ADORA2A in Human heart tissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA289458 staining ADORA2A in Human heart tissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysate from SH-SY5Y cell line, using ADORA2A Antibody (C-term). AAA289458 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from SH-SY5Y cell line, using ADORA2A Antibody (C-term). AAA289458 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-ADORA2A antibody
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Product Categories/Family for anti-ADORA2A antibody
References
Tiffany H.L.,et al.Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
Salvatore C.A.,et al.Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
Furlong T.J.,et al.Brain Res. Mol. Brain Res. 15:62-66(1992).
Le F.,et al.Biochem. Biophys. Res. Commun. 223:461-467(1996).
Puhl H.L. III,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Salvatore C.A.,et al.Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
Furlong T.J.,et al.Brain Res. Mol. Brain Res. 15:62-66(1992).
Le F.,et al.Biochem. Biophys. Res. Commun. 223:461-467(1996).
Puhl H.L. III,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44707
NCBI Official Full Name
adenosine receptor A2a
NCBI Official Synonym Full Names
adenosine A2a receptor
NCBI Official Symbol
ADORA2A
NCBI Official Synonym Symbols
A2aR; RDC8; ADORA2
NCBI Protein Information
adenosine receptor A2a
UniProt Protein Name
Adenosine receptor A2a
UniProt Gene Name
ADORA2A
UniProt Synonym Gene Names
ADORA2
UniProt Entry Name
AA2AR_HUMAN
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Product Notes
The ADORA2A adora2a (Catalog #AAA289458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADORA2A Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADORA2A can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ADORA2A adora2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADORA2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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