Rabbit ADORA2B Polyclonal Antibody | anti-ADORA2B antibody

ADORA2B Antibody

Cat. Num. AAA40578

• Gene Names: ADORA2B; ADORA2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: ADORA2B antibody is affinity chromatography purified via peptide column.
• Synonyms: ADORA2B, Antibody; ADORA2B Antibody; Adenosine receptor A2b; ADORA2; adenosine A2b receptor; anti-ADORA2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
• Purity/Purification: ADORA2B antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 332

• Applicable Applications for anti-ADORA2B antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: ADORA2B antibody was raised against a 19 amino acid peptide near the amino terminus of human ADORA2B.; The immunogen is located within the last 50 amino acids of ADORA2B.
• Positive Control: 1) - Mouse Kidney Tissue Lysate; 2) - Mouse Colon Tissue Lysate
• Preparation and Storage: ADORA2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of ADORA2B in rat colon tissue with ADORA2B antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of ADORA2B in rat colon tissue with ADORA2B antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of ADORA2B in mouse kidney tissue lysate with ADORA2B antibody at (A) 0.25 and (B) 0.5 μg/mL)

Related Product Information for anti-ADORA2B antibody

The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).

NCBI and Uniprot Product Information

• NCBI GI #: 4501951
• NCBI GeneID: 136
• NCBI Accession #: NP_000667
• NCBI GenBank Nucleotide #: NM_000676.2
• UniProt Accession #: P29275
• Molecular Weight: Predicted: 37 kDa; ; Observed: 41 kDa
• NCBI Official Full Name: adenosine receptor A2b
• NCBI Official Synonym Full Names: adenosine A2b receptor
• NCBI Official Symbol: ADORA2B
• NCBI Official Synonym Symbols: ADORA2
• NCBI Protein Information: adenosine receptor A2b
• UniProt Protein Name: Adenosine receptor A2b
• UniProt Gene Name: ADORA2B
• UniProt Entry Name: AA2BR_HUMAN