Rabbit ADORA3 / Adenosine A3 Receptor Polyclonal Antibody | anti-ADORA3 antibody

Rabbit Polyclonal to Human ADORA3 / Adenosine A3 Receptor

Cat. Num. AAA52213

• Gene Names: ADORA3; A3AR
• Reactivity: Dog, Horse, Human, Monkey, Pig
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: ADORA3 / Adenosine A3 Receptor, Antibody; Rabbit Polyclonal to Human ADORA3 / Adenosine A3 Receptor; Anti-ADORA3 / Adenosine A3 Receptor Antibody (Cytoplasmic Domain) IHC-plus; A3AR; AD026; Adenosine A3 receptor; BA552M11.5; A3 adenosine receptor; Adenosine receptor A3; RP11-552M11.7; Human ADORA3; Adenosine A3 Receptor; anti-ADORA3 antibody

• Host: Rabbit
• Reactivity: Dog, Horse, Human, Monkey, Pig
• Clonality: Polyclonal
• Specificity: Human ADORA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.6 mg/ml (varies by lot)
• Sequence Length: 318

• Applicable Applications for anti-ADORA3 antibody: IHC (Immunohistochemistry)
• Target: ADORA3/Adenosine A3 Receptor
• Immunogen Description: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human ADORA3. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Dog, Horse, Pig, Opossum (100%).
• Antigen Type: Synthetic peptide
• Immunogen: ADORA3 / Adenosine A3 Receptor antibody was raised against synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human ADORA3. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Dog, Horse, Pig, Opossum (100%).
• Antigen Modification: Cytoplasmic Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below. Can be stored undiluted at 4°C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-Adenosine A3 Receptor / ADORA3 antibody IHC of human tonsil, squamous epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20-30 ug/ml.)

Related Product Information for anti-ADORA3 antibody

A3 Adenosine Receptor is a widely expressed receptor that mediates the multiple functions of adenosine. For example, this receptor is believed to mediate the protective effect of adenosine released during cardiac ischemia. In lungs, the receptor mediates inhibition of eosinophil chemotaxis and may be useful in the treatment of eosinophil-dependent diseases such as asthma and rhinitis.

Product Categories/Family for anti-ADORA3 antibody

Family: GPCR; Subfamily: Adenosine

NCBI and Uniprot Product Information

• NCBI GI #: 4501953
• NCBI GeneID: 140
• NCBI Accession #: NP_000668.1
• NCBI GenBank Nucleotide #: NM_000677.3
• UniProt Accession #: P33765; Q6UWU0; Q9BYZ1; A2A3P4
• NCBI Official Full Name: adenosine receptor A3 isoform A
• NCBI Official Synonym Full Names: adenosine A3 receptor
• NCBI Official Symbol: ADORA3
• NCBI Official Synonym Symbols: A3AR
• NCBI Protein Information: adenosine receptor A3
• UniProt Protein Name: Adenosine receptor A3
• UniProt Gene Name: ADORA3
• UniProt Entry Name: AA3R_HUMAN