WB (Western Blot)
(Western blot analysis of extracts from MCF-7 cells, using AFAP1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit AFAP1 Polyclonal Antibody | anti-AFAP1 antibody
AFAP1 Antibody
Gene Names
AFAP1; AFAP; AFAP110; AFAP-110
Reactivity
Human, Mouse, RatPredicted: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
AFAP1, Antibody; AFAP1 Antibody; 110 kDa actin filament associated protein; 110 kDa actin filament-associated protein; Actin filament associated protein 1; Actin filament associated protein 110 kDa; Actin filament-associated protein 1; AFAP 1; AFAP 110; AFAP; AFAP-110; Afap1; AFAP1_HUMAN; AFAP110; FLJ56849; anti-AFAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Predicted: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
AFAP1 Antibody detects endogenous levels of AFAP1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
814
Applicable Applications for anti-AFAP1 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human AFAP1.
Tissue Specificity
Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Cytoskeleton
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from MCF-7 cells, using AFAP1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF-7 cells, using AFAP1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using AFAP1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using AFAP1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-AFAP1 antibody
Description: Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Post Translational Modifications: Phosphorylated on tyrosine residues by SRC.
Subunit Structure: Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers (By similarity). Interacts with activated SRC SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB and PRKCI (By similarity).
Post Translational Modifications: Phosphorylated on tyrosine residues by SRC.
Subunit Structure: Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers (By similarity). Interacts with activated SRC SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB and PRKCI (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 110 kDa
Predicted: 81 kDa
Predicted: 81 kDa
NCBI Official Full Name
actin filament-associated protein 1 isoform A
NCBI Official Synonym Full Names
actin filament associated protein 1
NCBI Official Symbol
AFAP1
NCBI Official Synonym Symbols
AFAP; AFAP110; AFAP-110
NCBI Protein Information
actin filament-associated protein 1
UniProt Protein Name
Actin filament-associated protein 1
UniProt Gene Name
AFAP1
UniProt Synonym Gene Names
AFAP; AFAP-110
UniProt Entry Name
AFAP1_HUMAN
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Product Notes
The AFAP1 afap1 (Catalog #AAA328763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AFAP1 Antibody reacts with Human, Mouse, Rat Predicted: Bovine, Horse, Sheep, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's AFAP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the AFAP1 afap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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