WB (Western Blot)
(Anti-AFP Picoband antibody, AAA45717-2.jpgAll lanes: Anti AFP (AAA45717) at 0.5ug/mlWB: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 69KDObserved bind size: 69KD)
Rabbit anti-Human, Mouse AFP Polyclonal Antibody | anti-AFP antibody
Anti-AFP Antibody
Gene Names
AFP; AFPD; FETA; HPAFP
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
AFP, Antibody; Anti-AFP Antibody; Alpha-fetoprotein; alpha-fetoprotein; AFP antibody; Alpha fetoglobulin antibody; Alpha fetoprotein antibody; Alpha fetoproteinprecursor antibody; Alpha-1-fetoprotein antibody; Alpha-fetoglobulin antibody; Alpha-fetoprotein antibody; alpha-fetoprotein, Hereditary persistence of, included antibody; FETA antibody; FETA_HUMAN antibody; Hereditary persistence of alpha fetoprotein antibody; HPAFP antibody; anti-AFP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
609
Applicable Applications for anti-AFP antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-AFP Picoband antibody, AAA45717-2.jpgAll lanes: Anti AFP (AAA45717) at 0.5ug/mlWB: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 69KDObserved bind size: 69KD)
WB (Western Blot)
(Anti-AFP Picoband antibody, AAA45717-2.jpgAll lanes: Anti AFP (AAA45717) at 0.5ug/mlWB: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 69KDObserved bind size: 69KD)
Application Data
(Anti-AFP Picoband antibody, AAA45717-1.jpgAll lanes: Anti AFP (AAA45717) at 0.5ug/mlWB: Human Recombinant AFP Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD)
Application Data
(Anti-AFP Picoband antibody, AAA45717-1.jpgAll lanes: Anti AFP (AAA45717) at 0.5ug/mlWB: Human Recombinant AFP Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD)
Related Product Information for anti-AFP antibody
Description: Rabbit IgG polyclonal antibody for Alpha-fetoprotein(AFP) detection. Tested with WB, IHC-P in Human, Mouse.
Background: AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a marker in biliary atresia, hepatitis, and yolk sac derived tumours.
Background: AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a marker in biliary atresia, hepatitis, and yolk sac derived tumours.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,678 Da
NCBI Official Full Name
Alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
AFPD; FETA; HPAFP
NCBI Protein Information
alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
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Product Notes
The AFP afp (Catalog #AAA45717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AFP Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AFP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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