IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody.)
Rabbit AFX Polyclonal Antibody | anti-AFX antibody
AFX (Phospho-Ser197) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
AFX, Antibody; AFX (Phospho-Ser197) Antibody; AFX; FOXO4; AFX1; Afxh; anti-AFX antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of AFX only when phosphorylated at serine 197.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
450
Applicable Applications for anti-AFX antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.
Modification
Phospho-Ser197
Target Name
AFX
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody.)
Related Product Information for anti-AFX antibody
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Product Categories/Family for anti-AFX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,941 Da
NCBI Official Full Name
forkhead box protein O4 isoform 2
NCBI Official Synonym Full Names
forkhead box O4
NCBI Official Symbol
FOXO4
NCBI Official Synonym Symbols
AFX; AFX1; MLLT7
NCBI Protein Information
forkhead box protein O4
UniProt Protein Name
Forkhead box protein O4
UniProt Gene Name
FOXO4
UniProt Synonym Gene Names
AFX; AFX1; MLLT7
UniProt Entry Name
FOXO4_HUMAN
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Product Notes
The AFX foxo4 (Catalog #AAA307418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AFX (Phospho-Ser197) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AFX can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AFX foxo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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