Rabbit anti-Human AGL Polyclonal Antibody | anti-AGL antibody

AGL Antibody (N-term)

Cat. Num. AAA290685

• Gene Names: AGL; GDE
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Synonyms: AGL, Antibody; AGL Antibody (N-term); Glycogen debranching enzyme; Glycogen debrancher; 4-alpha-glucanotransferase; Oligo-1;4-1;4-glucantransferase; Amylo-alpha-1;6-glucosidase; Amylo-1;6-glucosidase; Dextrin 6-alpha-D-glucosidase; AGL; GDE; anti-AGL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AGL.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Length: 1532

• Applicable Applications for anti-AGL antibody: ELISA, IF (Immunofluorescence), WB (Western Blot)
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus.
• Tissue Location: Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Expression of myc-GS causes wild type but not the „CBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL „CBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.)

WB (Western Blot)

(Anti-AGL Antibody (M15) at 1:1000 dilution + human skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 175 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-AGL antibody

Description: AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.

Background: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6- glucosidase in glycogen degradation.

Product Categories/Family for anti-AGL antibody

Primary Antibodies; Cancer; Signal Transduction

References

Horinishi, A., et al., J. Hum. Genet. 47 (2):55-59 (2002). Shen, J., et al., Hum. Mutat. 9 (1):37-40 (1997). Bao, Y., et al., Genomics 38 (2):155-165 (1996). Shen, J., et al., J. Clin. Invest. 98 (2):352-357 (1996). Yang, B.Z., et al., J. Biol. Chem. 267 (13):9294-9299 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 116734847
• NCBI GeneID: 178
• NCBI Accession #: NP_000019.2
• NCBI GenBank Nucleotide #: NM_000028.2
• UniProt Accession #: P35573; P78354; P78544; Q59H92; Q6AZ90; Q9UF08; A6NCX7; A6NEK2; D3DT51
• Molecular Weight: 174764 Da
• NCBI Official Full Name: glycogen debranching enzyme isoform 1
• NCBI Official Synonym Full Names: amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
• NCBI Official Symbol: AGL
• NCBI Official Synonym Symbols: GDE
• NCBI Protein Information: glycogen debranching enzyme
• UniProt Protein Name: Glycogen debranching enzyme
• UniProt Gene Name: AGL
• UniProt Synonym Gene Names: GDE; Amylo-1,6-glucosidase
• UniProt Entry Name: GDE_HUMAN