WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells, using AHCYL1 antibody.)
Rabbit AHCYL1 Polyclonal Antibody | anti-AHCYL1 antibody
AHCYL1 Antibody
Gene Names
AHCYL1; DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
AHCYL1, Antibody; AHCYL1 Antibody; AA409031; AdoHcyase 2; Ahcy-rs3; AHCYL1; DC expressed AHCY like molecule; DC-expressed AHCY-like molecule; DCAL; Dendritic cell expressed AHCY like protein; Inositol 1, 4, 5-trisphosphate receptor-binding protein; IP3R binding protein released with inositol 1, 4, 5-trisphosphate; IRBIT; PRO0233; Putative adenosylhomocysteinase 2; S adenosyl L homocysteine hydrolase 2; S adenosylhomocysteine hydrolase like 1; S-adenosyl-L-homocysteine hydrolase 2; S-adenosylhomocysteine hydrolase-like protein 1; SAHH2_HUMAN; XPVKONA; anti-AHCYL1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AHCYL1 antibody detects endogenous levels of AHCYL1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-AHCYL1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human AHCYL1.
Subcellular Location
Endoplasmic Reticulum; Cytosol; Plasma Membrane
Tissue Specificity
Expressed in dendritic cells.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells, using AHCYL1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells, using AHCYL1 antibody.)
IHC (Immunohiostchemistry)
(AAA322917 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA322917 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from rat spleen, using AHCYL1 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from rat spleen, using AHCYL1 antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-AHCYL1 antibody
Function: Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3-and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1, 4, 5-trisphosphate competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. In the pancreatic and salivary ducts, at resting state, attenuates inositol 1, 4, 5-trisphosphate-induced calcium release by interacting with ITPR1 (PubMed:16793548). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1, 4, 5-trisphosphate production, dissociates of ITPR1 to interact with CFTR and SLC26A6 mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (By similarity). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3-secretion (PubMed:16769890). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (By similarity). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2 (PubMed:20584908). Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition (PubMed:18829453). May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state (PubMed:19224921). Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (PubMed:25237103). In vitro does not exhibit any S-adenosyl-L-homocysteine hydrolase activity (By similarity).
Subunit Structure: Forms multimers (PubMed:16793548). Forms heteromultimers with AHCYL2 (via the C-terminal region) (PubMed:19220705). Interacts (when phosphorylated) with ITPR1 (when not phosphorylated); the interaction suppresses inositol 1, 4, 5-trisphosphate binding to ITPR1 (PubMed:16793548). Interacts with CFTR and SLC26A6; the interactions take place once AHCYL1 is released from ITPR1 and increase CFTR and SLC26A6 activities (By similarity). Interacts with RRM1; in a phosphorylation-and (dATP)-dependent manner. Interacts (via PEST domain when phosphorylated) with SLC4A4 isoform 1 but not isoform 2; the interaction increases SLC4A4 isoform 1 activity (PubMed:16769890). Interacts (when phosphorylated) with SLC9A3; the interaction is required for SLC9A3 apical location and activity (PubMed:18829453, PubMed:20584908). Interacts (when phosphorylated) with FIP1L1; the interaction is direct and associates AHCYL1 with the CPSF complex and RNA (PubMed:19224921). Interacts with PAPOLA (PubMed:19224921).
Post-translational Modifications: Phosphorylated at Ser/Thr residues between Ser-68 and Thr-72 in the PEST region: required for interaction with dATP-bound RRM1 and ITPR1. Phosphorylation at Ser-68 by PRKD1 and CAMK4 is required for further phosphorylations by CSNK1A1 (PubMed:16793548). Phosphorylation is induced by oxidative stress (PubMed:19224921). Probably phosphorylated by CAMK2A; phosphorylation at Ser-68 may be required for interaction with SLC9A3 (PubMed:20584908).
Similarity: The PEST region is essential for the interaction with ITPR1, and, when phosphorylated, is also the RRM1-binding region. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1 (By similarity). Belongs to the adenosylhomocysteinase family.
Subunit Structure: Forms multimers (PubMed:16793548). Forms heteromultimers with AHCYL2 (via the C-terminal region) (PubMed:19220705). Interacts (when phosphorylated) with ITPR1 (when not phosphorylated); the interaction suppresses inositol 1, 4, 5-trisphosphate binding to ITPR1 (PubMed:16793548). Interacts with CFTR and SLC26A6; the interactions take place once AHCYL1 is released from ITPR1 and increase CFTR and SLC26A6 activities (By similarity). Interacts with RRM1; in a phosphorylation-and (dATP)-dependent manner. Interacts (via PEST domain when phosphorylated) with SLC4A4 isoform 1 but not isoform 2; the interaction increases SLC4A4 isoform 1 activity (PubMed:16769890). Interacts (when phosphorylated) with SLC9A3; the interaction is required for SLC9A3 apical location and activity (PubMed:18829453, PubMed:20584908). Interacts (when phosphorylated) with FIP1L1; the interaction is direct and associates AHCYL1 with the CPSF complex and RNA (PubMed:19224921). Interacts with PAPOLA (PubMed:19224921).
Post-translational Modifications: Phosphorylated at Ser/Thr residues between Ser-68 and Thr-72 in the PEST region: required for interaction with dATP-bound RRM1 and ITPR1. Phosphorylation at Ser-68 by PRKD1 and CAMK4 is required for further phosphorylations by CSNK1A1 (PubMed:16793548). Phosphorylation is induced by oxidative stress (PubMed:19224921). Probably phosphorylated by CAMK2A; phosphorylation at Ser-68 may be required for interaction with SLC9A3 (PubMed:20584908).
Similarity: The PEST region is essential for the interaction with ITPR1, and, when phosphorylated, is also the RRM1-binding region. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1 (By similarity). Belongs to the adenosylhomocysteinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
S-adenosylhomocysteine hydrolase-like protein 1 isoform b
NCBI Official Synonym Full Names
adenosylhomocysteinase like 1
NCBI Official Symbol
AHCYL1
NCBI Official Synonym Symbols
DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA
NCBI Protein Information
S-adenosylhomocysteine hydrolase-like protein 1
UniProt Protein Name
S-adenosylhomocysteine hydrolase-like protein 1
UniProt Gene Name
AHCYL1
UniProt Synonym Gene Names
DCAL; IRBIT; XPVKONAImportedManual assertion inferred from database entriesiHGNC:344; IRBIT; AdoHcyase 2
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Product Notes
The AHCYL1 ahcyl1 (Catalog #AAA322917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AHCYL1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AHCYL1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AHCYL1 ahcyl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHCYL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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