<
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Goat AIF1/IBA1 Polyclonal Antibody | anti-AIF1 antibody
Goat anti-AIF1/IBA1 (isoform 3) Antibody
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Dog
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
AIF1/IBA1, Antibody; Goat anti-AIF1/IBA1 (isoform 3) Antibody; Goat Anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody; AIF1; IBA1; allograft inflammatory factor 1; DASS-82G15.2; AIF-1; IRT-1; Em: AF129756.17; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; AIF1 antibody; IBA1 antibody; allograft inflammatory factor 1 antibody; DASS-82G15.2 antibody; AIF-1 antibody; IRT-1 antibody; Em: AF129756.17 antibody; interferon gamma responsive transcript antibody; ionized calcium-binding adapter molecule antibody; AIF1 / IBA1 (isoform 3); anti-AIF1 antibody
Host
Goat
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog
Expected from sequence similarity: Human, Mouse, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
NKQFLDDPKYSSDED
Sequence Length
147
Applicable Applications for anti-AIF1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes
ELISA: Antibody detection limit dilution 1:128000.
IHC: In paraffin embedded human lung. Recommended concentration: 6µg/ml.
FC: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
IF: Strong expression of the protein seen in the cytoplasm of U937 and CaCo-2 cells. Recommended concentration: 10µg/ml.
IHC: In paraffin embedded human lung. Recommended concentration: 6µg/ml.
FC: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
IF: Strong expression of the protein seen in the cytoplasm of U937 and CaCo-2 cells. Recommended concentration: 10µg/ml.
Immunogen
Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.
Note
This antibody is expected to recognise isoform 3 (NP_001614.3) only.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA13659 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA13659 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.)
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.)
IHC (Immunohistochemistry)
((6µg/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
((6µg/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
WB (Western Blot)
((0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
allograft inflammatory factor 1 isoform 3
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
AIF1
NCBI Official Synonym Symbols
IBA1; IRT1; AIF-1; IRT-1
NCBI Protein Information
allograft inflammatory factor 1
UniProt Protein Name
Allograft inflammatory factor 1
UniProt Gene Name
AIF1
UniProt Synonym Gene Names
G1; IBA1; AIF-1
UniProt Entry Name
AIF1_HUMAN
Similar Products
Product Notes
The AIF1 aif1 (Catalog #AAA13659) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AIF1/IBA1 (isoform 3) Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's AIF1/IBA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC). ELISA: Antibody detection limit dilution 1:128000. IHC: In paraffin embedded human lung. Recommended concentration: 6µg/ml. FC: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml. IF: Strong expression of the protein seen in the cytoplasm of U937 and CaCo-2 cells. Recommended concentration: 10µg/ml. Researchers should empirically determine the suitability of the AIF1 aif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKQFLDDPKY SSDED. It is sometimes possible for the material contained within the vial of "AIF1/IBA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.