FCM/FACS (Flow Cytometry)
(AIFM2 Antibody (C-term) (AAA285125) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit AIFM2 Polyclonal Antibody | anti-AIFM2 antibody
AIFM2 Antibody (C-term)
Gene Names
AIFM2; AMID; PRG3
Reactivity
Human(Predicted Reactivity: Bovine, Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AIFM2, Antibody; AIFM2 Antibody (C-term); Apoptosis-inducing factor 2; 1---; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; p53-responsive gene 3 protein; AIFM2; AMID; PRG3 {ECO:0000303|PubMed:12135761}; anti-AIFM2 antibody
Host
Rabbit
Reactivity
Human
(Predicted Reactivity: Bovine, Mouse)
(Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AIFM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human AIFM2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
319-348
Sequence Length
336
Applicable Applications for anti-AIFM2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
A NAD(P)H-dependent oxidoreductase involved in cellular oxidative stress response. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilicradical- trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels (PubMed: , PubMed: ). May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death (PubMed: ). Capable of DNA binding in a non-sequence specific way (PubMed: ).
Cellular Location
Lipid droplet. Cell membrane; Lipid-anchor Cytoplasm. Mitochondrion membrane. Nucleus
Tissue Location
Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(AIFM2 Antibody (C-term) (AAA285125) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(AIFM2 Antibody (C-term) (AAA285125) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AIFM2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AIFM2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of AIFM2 Antibody (C-term) (AAA285125) in mouse liver tissue lysates (35ug/lane). AIFM2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of AIFM2 Antibody (C-term) (AAA285125) in mouse liver tissue lysates (35ug/lane). AIFM2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-AIFM2 antibody
AIFM2 is significant homology to NADH oxidoreductases and the apoptosis-inducing factor PDCD8/AIF. The protein has been shown to induce apoptosis. This protein is found to be induced by tumor suppressor protein p53 in colon caner cells.
Product Categories/Family for anti-AIFM2 antibody
References
Ohiro Y., Garkavtsev I.FEBS Lett. 524:163-171(2002)
Wu M., Xu L.-G., Su T.Oncogene 23:6815-6819(2004)
Wu M., Xu L.-G., Su T.Oncogene 23:6815-6819(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40527
NCBI Official Full Name
apoptosis-inducing factor 2
NCBI Official Synonym Full Names
apoptosis-inducing factor, mitochondrion-associated, 2
NCBI Official Symbol
AIFM2
NCBI Official Synonym Symbols
AMID; PRG3
NCBI Protein Information
apoptosis-inducing factor 2
UniProt Protein Name
Apoptosis-inducing factor 2
UniProt Gene Name
AIFM2
UniProt Entry Name
AIFM2_HUMAN
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Product Notes
The AIFM2 aifm2 (Catalog #AAA285125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-348. The AIFM2 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's AIFM2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AIFM2 aifm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIFM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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