Rabbit AKR1A1 Polyclonal Antibody | anti-AKR1A1 antibody

AKR1A1 Antibody (C-term)

Cat. Num. AAA283606

• Gene Names: AKR1A1; ALR; ARM; DD3; ALDR1; HEL-S-6
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: AKR1A1, Antibody; AKR1A1 Antibody (C-term); Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Aldo-keto reductase family 1 member A1; AKR1A1; ALDR1; ALR; anti-AKR1A1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This AKR1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-325 amino acids from the C-terminal region of human AKR1A1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 293-325
• Sequence Length: 325

• Applicable Applications for anti-AKR1A1 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Function: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (PubMed:10510318). Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN) (PubMed:18276838, PubMed:11306097). Displays no reductase activity towards retinoids (By similarity).
• Cellular Location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9JII6}. Apical cell membrane {ECO:0000250|UniProtKB:Q9JII6}
• Tissue Location: Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.)

WB (Western Blot)

(Western blot analysis of anti-AKR1A1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). AKR1A1(arrow) was detected using the purified Pab.)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-AKR1A1 antibody

AKR1A1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

Product Categories/Family for anti-AKR1A1 antibody

Cell Biology; Metabolism; Signal transduction

References

Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008)
Bohren,K.M.,Biochim. Biophys. Acta 1748 (2), 201-212 (2005)
El-Kabbani,O.,Acta Crystallogr. D Biol. Crystallogr. 50 (PT 6), 859-868 (1994)

NCBI and Uniprot Product Information

• NCBI GI #: 320202988
• NCBI GeneID: 10327
• NCBI Accession #: NP_001189342.1; NP_001189343.1; NP_006057.1; NP_697021.1
• NCBI GenBank Nucleotide #: NM_001202413.1
• UniProt Accession #: P14550; Q6IAZ4; A8KAL8; D3DQ04
• Molecular Weight: 36573
• NCBI Official Full Name: alcohol dehydrogenase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member A1 (aldehyde reductase)
• NCBI Official Symbol: AKR1A1
• NCBI Official Synonym Symbols: ALR; ARM; DD3; ALDR1; HEL-S-6
• NCBI Protein Information: alcohol dehydrogenase [NADP(+)]
• UniProt Protein Name: Alcohol dehydrogenase [NADP(+)]
• UniProt Gene Name: AKR1A1
• UniProt Synonym Gene Names: ALDR1; ALR
• UniProt Entry Name: AK1A1_HUMAN