IHC (Immunohiostchemistry)
(immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Rabbit AKT1/AKT2/AKT3 Polyclonal Antibody | anti-AKT1/AKT2/AKT3 antibody
AKT1/AKT2/AKT3 antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
AKT1/AKT2/AKT3 antibody was purified by immunoaffinity chromatography.
Synonyms
AKT1/AKT2/AKT3, Antibody; AKT1/AKT2/AKT3 antibody; Polyclonal AKT1/AKT2/AKT3; Anti-AKT1/AKT2/AKT3; AKT1/AKT2/AKT3; AKT/AKT2/AKT3-1; AKT/AKT2/AKT3 1; Phospho Tyr315/316/312; anti-AKT1/AKT2/AKT3 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
AKT1/AKT2/AKT3 antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-AKT1/AKT2/AKT3 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
Biological Significance
Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8. Akt2 is an important signaling molecule in the Insulin signaling pathway. It is required to induce glucose transport. In a mouse which is null for Akt1 but normal for Akt2, glucose homeostasis is unperturbed, but the animals are smaller, consistent with a role for Akt1 in growth. In contrast, mice which do not have Akt2, but have normal Akt1, have mild growth deficiency and display a diabetic phenotype (insulin resistance), again consistent with the idea that Akt2 is more specific for the insulin receptor signaling pathway. The role of Akt3 is less clear, though it appears to be predominantly expressed in brain. It has been reported that mice lacking Akt3 have small brains.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
AKT1/AKT2/AKT3 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohiostchemistry)
(immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) antibody (left) or the same antibody preincubated with with blocking peptide (right))
IHC (Immunohiostchemistry)
(immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) antibody (left) or the same antibody preincubated with with blocking peptide (right))
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using AKT1/AKT2/AKT3(Phospho-Tyr315/316/312) antibody)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using AKT1/AKT2/AKT3(Phospho-Tyr315/316/312) antibody)
Related Product Information for anti-AKT1/AKT2/AKT3 antibody
Rabbit polyclonal AKT1/AKT2/AKT3 antibody
Product Categories/Family for anti-AKT1/AKT2/AKT3 antibody
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Product Notes
The AKT1/AKT2/AKT3 (Catalog #AAA107692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AKT1/AKT2/AKT3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the AKT1/AKT2/AKT3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT1/AKT2/AKT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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