IHC (Immunohiostchemistry)
(Mouse Akt1 Antibody (N-term) (AAA288066)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Akt1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit Akt1 Polyclonal Antibody | anti-Akt1 antibody
Mouse Akt1 Antibody (N-term)
Gene Names
Akt1; Akt; PKB; Rac; PKB/Akt; PKBalpha
Reactivity
Mouse (Predicted Reactivity: Human)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Akt1, Antibody; Mouse Akt1 Antibody (N-term); RAC-alpha serine/threonine-protein kinase; AKT1 kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; Thymoma viral proto-oncogene; Akt1; Akt; Rac; anti-Akt1 antibody
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Human)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
22-49
Sequence Length
488
Applicable Applications for anti-Akt1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Mouse Akt1 Antibody (N-term) (AAA288066)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Akt1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Mouse Akt1 Antibody (N-term) (AAA288066)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Akt1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Mouse Akt1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Akt1 antibody detected the Akt1 protein (arrow).)
WB (Western Blot)
(Mouse Akt1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Akt1 antibody detected the Akt1 protein (arrow).)
Related Product Information for anti-Akt1 antibody
This gene encodes the founding member of the Akt
serine-threonine protein kinase gene family that also includes Akt2
and Akt3. This kinase is a major downstream effector of the
phosphatidylinositol 3-kinase (PI3K) pathway that mediates the
effects of various growth factors such as platelet-derived growth
factor (PDGF), epidermal growth factor (EGF), insulin and
insulin-like growth factor I (IGF-I). It is activated through
recruitment to cellular membranes by PI3K lipid products and by
phosphorylation by 3-phosphoinositide dependent kinase-1. It then
further phosphorylates different downstream proteins in response to
various extracellular signals and thus plays a pivotal role in
mediating a variety of cellular processes, such as glucose
metabolism, glycogen biosynthesis, protein synthesis and turn over,
inflammatory response, cell survival (anti-apoptosis) and
development. Alternatively spliced transcript variants encoding
different isoforms have been found for this gene. [provided by
RefSeq].
serine-threonine protein kinase gene family that also includes Akt2
and Akt3. This kinase is a major downstream effector of the
phosphatidylinositol 3-kinase (PI3K) pathway that mediates the
effects of various growth factors such as platelet-derived growth
factor (PDGF), epidermal growth factor (EGF), insulin and
insulin-like growth factor I (IGF-I). It is activated through
recruitment to cellular membranes by PI3K lipid products and by
phosphorylation by 3-phosphoinositide dependent kinase-1. It then
further phosphorylates different downstream proteins in response to
various extracellular signals and thus plays a pivotal role in
mediating a variety of cellular processes, such as glucose
metabolism, glycogen biosynthesis, protein synthesis and turn over,
inflammatory response, cell survival (anti-apoptosis) and
development. Alternatively spliced transcript variants encoding
different isoforms have been found for this gene. [provided by
RefSeq].
Product Categories/Family for anti-Akt1 antibody
References
Chang, Z., et al. Dev. Biol. 347(2):384-391(2010)
Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010)
Sun, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(39):16887-16892(2010)
Goncalves, M.D., et al. PLoS ONE 5 (9), E12707 (2010) :
Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010) :
Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010)
Sun, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(39):16887-16892(2010)
Goncalves, M.D., et al. PLoS ONE 5 (9), E12707 (2010) :
Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55707
NCBI Official Full Name
RAC-alpha serine/threonine-protein kinase isoform 2
NCBI Official Synonym Full Names
thymoma viral proto-oncogene 1
NCBI Official Symbol
Akt1
NCBI Official Synonym Symbols
Akt; PKB; Rac; PKB/Akt; PKBalpha
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
UniProt Gene Name
Akt1
UniProt Synonym Gene Names
Akt; Rac; PKB; PKB alpha
UniProt Entry Name
AKT1_MOUSE
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Product Notes
The Akt1 akt1 (Catalog #AAA288066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-49. The Mouse Akt1 Antibody (N-term) reacts with Mouse (Predicted Reactivity: Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Akt1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Akt1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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