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IHC (Immunohistochemistry)
(AKT2 Antibody (Center) (AAA288563)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human AKT2 Polyclonal Antibody | anti-AKT2 antibody
AKT2 Antibody (Center)
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AKT2, Antibody; AKT2 Antibody (Center); RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; anti-AKT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human AKT2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
93-123
Sequence Length
481
Applicable Applications for anti-AKT2 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(AKT2 Antibody (Center) (AAA288563)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(AKT2 Antibody (Center) (AAA288563)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(AKT2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(AKT2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of AKT2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of AKT2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(AKT2 Antibody (N108) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).)
WB (Western Blot)
(AKT2 Antibody (N108) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).)
WB (Western Blot)
(Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-AKT2 antibody
AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Product Categories/Family for anti-AKT2 antibody
References
Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003).
Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003).
Jones, D.T., et al., Blood 101(8):3174-3180 (2003).
Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003).
Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).
Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003).
Jones, D.T., et al., Blood 101(8):3174-3180 (2003).
Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003).
Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55769
NCBI Official Full Name
RAC-beta serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
NCBI Protein Information
RAC-beta serine/threonine-protein kinase
UniProt Protein Name
RAC-beta serine/threonine-protein kinase
UniProt Gene Name
AKT2
UniProt Synonym Gene Names
PKB beta
UniProt Entry Name
AKT2_HUMAN
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Product Notes
The AKT2 akt2 (Catalog #AAA288563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 93-123. The AKT2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKT2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AKT2 akt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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