IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit AlaRS (AARS) Polyclonal Antibody | anti-AARS antibody
AlaRS (AARS) Antibody (N-term)
Gene Names
AARS; CMT2N; EIEE29
Reactivity
Human (Predicted Reactivity: C Elegans, Drosophila, Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AlaRS (AARS), Antibody; AlaRS (AARS) Antibody (N-term); Alanine--tRNA ligase; cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}; 6117 {ECO:0000255|HAMAP-Rule:MF_03133}; Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}; AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}; Renal carcinoma antigen NY-REN-42; AARS {ECO:0000255|HAMAP-Rule:MF_03133}; anti-AARS antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: C Elegans, Drosophila, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AlaRS (AARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human AlaRS (AARS).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
61-91
Sequence Length
968
Applicable Applications for anti-AARS antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Related Product Information for anti-AARS antibody
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
References
Shiba,K.,Biochemistry 34 (33), 10340-10349 (1995)
Ripmaster,T.L.,Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995)
Sang Lee,J.,Cell 127 (3), 635-648 (2006)
Ripmaster,T.L.,Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995)
Sang Lee,J.,Cell 127 (3), 635-648 (2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106810
NCBI Official Full Name
alanine--tRNA ligase, cytoplasmic
NCBI Official Synonym Full Names
alanyl-tRNA synthetase
NCBI Official Symbol
AARS
NCBI Official Synonym Symbols
CMT2N; EIEE29
NCBI Protein Information
alanine--tRNA ligase, cytoplasmic
UniProt Protein Name
Alanine--tRNA ligase, cytoplasmic
UniProt Gene Name
AARS
UniProt Synonym Gene Names
AlaRS
UniProt Entry Name
SYAC_HUMAN
Similar Products
Product Notes
The AARS aars (Catalog #AAA284649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 61-91. The AlaRS (AARS) Antibody (N-term) reacts with Human (Predicted Reactivity: C Elegans, Drosophila, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's AlaRS (AARS) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AARS aars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AlaRS (AARS), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.